Key Soil Abiotic Factors Driving Soil Sickness in Lycium barbarum L. Under Long-Term Monocropping

Sustainable cultivation of Lycium barbarum L. (L. barbarum) in northwest China faces challenges due to soil sickness. While previous studies have explored variations in L. barbarum's root-associated microbiota, the impact of soil properties on its growth performance and plant-soil feedback remains unclear. This study investigated changes in soil properties across topsoil (0-20 cm) and subsoil (20-40 cm) in primary L. barbarum cultivation regions of northwest China, evaluating seedling growth and plant-soil feedback through pot experiments. Results revealed significantly higher fresh shoot weights in seedlings cultivated in topsoil compared to subsoil, with plant-soil feedback showing an inverse trend. Redundancy analysis indicated positive correlations between both fresh weight and plant-soil feedback with electrical conductivity and dissolved nitrogen content, while negative correlations were observed with soil pH at both depths. Notably, dissolved organic carbon content negatively correlated with fresh weight and plant-soil feedback in topsoil, suggesting a potential relationship between continuous single-species plant litter input and soil sickness under monocropping conditions. These findings indicate that long-term input of a single plant litter type, rather than chemical fertilization, may primarily contribute to L. barbarum soil sickness in northwest China, providing valuable insights for developing sustainable cultivation practices for growing L. barbarum.