Highly Efficient Genome Editing of Human Primary Immune Cells Using Scalable Microfluidic Droplet-Enabled Cell Mechanoporator

被引：0
|
作者：
Joo, Byeongju [1 ]
Kim, You-Jeong [2 ,3 ]
Yun, Dayoung [1 ,4 ]
Im, Sungbin [1 ]
Jung, Cheulhee [4 ]
Chung, Aram [1 ,2 ,3 ]
机构：
[1] MxT Biotech, Seoul, South Korea
[2] Korea Univ, Sch Biomed Engn, Seoul, South Korea
[3] Korea Univ, Interdisciplinary Program Precis Publ Hlth, Seoul, South Korea
[4] Korea Univ, Dept Biotechnol, Seoul, South Korea
来源：
MOLECULAR THERAPY | 2024年 / 32卷 / 04期
关键词：
D O I：
暂无
中图分类号：
Q81 [生物工程学（生物技术）]; Q93 [微生物学];
学科分类号：
071005 ; 0836 ; 090102 ; 100705 ;
摘要：
735
引用
收藏
页码：370 / 370
页数：1
相关论文
共 50 条
  • [21] Phenotyping polarization dynamics of immune cells using a lipid droplet-cell pairing microfluidic platform
    Pinon, Lea
    Ruyssen, Nicolas
    Pineau, Judith
    Mesdjian, Olivier
    Cuvelier, Damien
    Chipont, Anna
    Allena, Rachele
    Guerin, Coralie L.
    Asnacios, Sophie
    Asnacios, Atef
    Pierobon, Paolo
    Fattaccioli, Jacques
    CELL REPORTS METHODS, 2022, 2 (11):
  • [22] Precision Genome Editing: Identification of Highly Efficient and Specific Guides for CRISPR-Cas9 Gene Editing in Human T Cell Loci
    Garner, Elizabeth
    Donohoue, Paul
    Lau, Elaine
    Vidal, Bastien
    Settle, Alex
    Irby, Matthew
    Rotstein, Tomer
    Banh, Lynda
    Toh, Mckenzi
    Williams, Carolyn
    Smith, Stephen
    Gradia, Scott
    Stengel, Katharina
    Kohrs, Bryan
    Fuller, Christopher
    Kennedy, Rachel
    Eshghi, Shawdee
    Slorach, Euan
    van Overbeek, Megan
    May, Andrew
    Kanner, Steven
    MOLECULAR THERAPY, 2019, 27 (04) : 388 - 388
  • [23] Highly efficient genome editing of human hematopoietic stem cells via a nano-silicon-blade delivery approach
    Ma, Yuan
    Han, Xin
    Quintana Bustamante, Oscar
    Bessa de Castro, Ricardo
    Zhang, Kai
    Zhang, Pengchao
    Li, Ying
    Liu, Zongbin
    Liu, Xuewu
    Ferrari, Mauro
    Hu, Zhongbo
    Segovia, Jose Carlos
    Qin, Lidong
    INTEGRATIVE BIOLOGY, 2017, 9 (06) : 548 - 554
  • [24] Highly efficient RNA-guided genome editing in human cells via delivery of purified Cas9 ribonucleoproteins
    Kim, Sojung
    Kim, Daesik
    Cho, Seung Woo
    Kim, Jungeun
    Kim, Jin-Soo
    GENOME RESEARCH, 2014, 24 (06) : 1012 - 1019
  • [25] The CRISPR-Cas12a Platform for Accurate Genome Editing, Gene Disruption, and Efficient Transgene Integration in Human Immune Cells
    Mohr, Marina
    Damas, Nkerorema
    Gudmand-Hoyer, Johanne
    Zeeberg, Katrine
    Jedrzejczyk, Dominika
    Vlassis, Arsenios
    Morera-Gomez, Marti
    Pereira-Schoning, Sara
    Pus, Urska
    Oliver-Almirall, Anna
    Jensen, Tanja Lyholm
    Baumgartner, Roland
    Weinert, Brian Tate
    Gill, Ryan T.
    Warnecke, Tanya
    ACS SYNTHETIC BIOLOGY, 2023, 12 (02): : 375 - 389
  • [26] Continuous Production of Highly Functional Vascularized Hepatobiliary Organoids from Human Pluripotent Stem Cells using a Scalable Microfluidic Platform
    Abbasalizadeh, Saeed
    Babaee, Sahab
    Kowsari-Esfahan, Reza
    Mazidi, Zahra
    Shi, Yichao
    Wainer, Jake
    Cabral, Joaquim M. S.
    Langer, Robert
    Traverso, Giovanni
    Baharvand, Hossein
    ADVANCED FUNCTIONAL MATERIALS, 2023, 33 (49)
  • [27] A Tri-Droplet Liquid Structure for Highly Efficient Intracellular Delivery in Primary Mammalian Cells Using Digital Microfluidics
    Little, Samuel R.
    Leung, Ziuwin
    Quach, Angela B. V.
    Hirukawa, Alison
    Gholizadeh, Fatemeh
    Hajiaghayi, Mehri
    Darlington, Peter J.
    Shih, Steve C. C.
    ADVANCED MATERIALS TECHNOLOGIES, 2023,
  • [28] A Tri-Droplet Liquid Structure for Highly Efficient Intracellular Delivery in Primary Mammalian Cells Using Digital Microfluidics
    Little, Samuel R.
    Leung, Ziuwin
    Quach, Angela B. V.
    Hirukawa, Alison
    Gholizadeh, Fatemeh
    Hajiaghayi, Mehri
    Darlington, Peter J.
    Shih, Steve C. C.
    ADVANCED MATERIALS TECHNOLOGIES, 2023, 8 (21)
  • [29] Highly efficient non-viral delivery of macromolecules to unactivated human T cells using microfluidic transfection
    Chehal, Manreet
    Ouellet, Eric
    Binson, Elinor
    Nguyen, Quan
    Liao, Tina
    Chau, Phillip
    Chen, Kris
    Lambertz, Ulrike
    Yu, Jessie
    Chen, Kevin
    Loughhead, Scott
    Dalton, Bob
    Eaves, Allen
    Louis, Sharon
    Kokaji, Andy
    JOURNAL OF IMMUNOLOGY, 2024, 212 (01):
  • [30] Single cell transfection of human-induced pluripotent stem cells using a droplet-based microfluidic system
    Perez-Sosa, Camilo
    Sanluis-Verdes, Anahi
    Waisman, Ariel
    Lombardi, Antonella
    Rosero, Gustavo
    La Greca, Alejandro
    Bhansali, Shekhar
    Bourguignon, Natalia
    Luzzani, Carlos
    Perez, Maximiliano S.
    Miriuka, Santiago
    Lerner, Betiana
    ROYAL SOCIETY OPEN SCIENCE, 2022, 9 (01):
12345