Synthesis of α-D-Ribose 1-Phosphate and 2-Deoxy-α-D-Ribose 1-Phosphate Via Enzymatic Phosphorolysis of 7-Methylguanosine and 7-Methyldeoxyguanosine

被引：4
|
作者：
Varizhuk, Irina V. [1 ]
Oslovsky, Vladimir E. [1 ]
Solyev, Pavel N. [1 ]
Drenichev, Mikhail S. [1 ]
Mikhailov, Sergey N. [1 ]
机构：
[1] Russian Acad Sci, Engelhardt Inst Mol Biol, Moscow, Russia
来源：
CURRENT PROTOCOLS | 2022年 / 2卷 / 01期
基金：
俄罗斯科学基金会;
关键词：
2-deoxyribose; 1-phosphate; enzymatic synthesis of nucleosides; nucleoside phosphorylases; ribose; PURINE-NUCLEOSIDE PHOSPHORYLASE; ESCHERICHIA-COLI;
D O I：
10.1002/cpz1.347
中图分类号：
Q5 [生物化学];
学科分类号：
071010 ; 081704 ;
摘要：
A simple and efficient method for the preparation of alpha-D-ribose 1-phosphate and 2-deoxy-alpha-D-ribose 1-phosphate, key intermediates in nucleoside metabolism and important starting compounds for the enzymatic synthesis of various modified nucleosides, has been proposed. It consists in near-irreversible enzymatic phosphorolysis of readily prepared hydroiodide salts of 7-methylguanosine and 7-methyl-2-deoxyguanosine, respectively, in the presence of purine nucleoside phosphorylase. alpha-D-Ribose 1-phosphate and 2-deoxy-alpha-D-ribose 1-phosphate are obtained in near quantitative yields (by HPLC analysis) and 74%-94% yields after their isolation and purification. (c) 2022 Wiley Periodicals LLC.
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页数：16
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