The Batailing Au deposit is a vein-type deposit in central Jilin Province, situated in the southern sector of the Lesser Xing'an-Zhangguangcai Range within the eastern Central Asian Orogenic Belt. NE-trending fault-controlled orebodies occur in the Upper Permian Yangjiagou Formation and quartz diorite-porphyrite. The mineralisation process was delineated into three stages: (I) quartz-arsenopyrite-pyrite, (II) quartz-polymetallic sulphides (main Au mineralisation stage), and (III) quartz-pyrite-carbonate. Fluid inclusions (FIs) in quartz were identified as four types: PC-type (pure CO2), C1-type (CO2-bearing), C2-type (CO2-rich), and W-type (aqueous two-phase). Raman spectroscopy analysis revealed that the vapor components of the FIs predominantly comprised CO2 with minor quantities of CH4 in stages I-II. Stages I and II encompassed four types of FIs with homogenisation temperature ranging from 264 to 332 degrees C and 213 to 292 degrees C and salinity spanning from 4.7 to 11.2 wt% and 1.8 to 11.6 wt%, respectively. Stage III exclusively contained W-type FIs with homogenisation temperature ranging from 152 to 215 degrees C and salinity spanning from 1.4 to 6.4 wt%. H-O isotopic values (delta D = -84 to -79.6 parts per thousand, delta 18OH2O = 6.2 to 6.4 parts per thousand in stage I and delta D = -96.4 to -90.4 parts per thousand, delta 18OH2O = 2.8 to 4.4 parts per thousand in stage II) and microthermometric data indicated that the ore-forming fluids are initially from a magmatic source, with later meteoric water input. Low C isotopic data from CO2 in FIs in quartz (-24.4 to -24.3 parts per thousand in stage I and -23.7 to -22.6 parts per thousand in stage II) indicated an organic carbon source. Ore precipitation is mainly attributable to fluid immiscibility. S-Pb isotopic data (delta 34S = -3.5 to -1.6 parts per thousand; 206Pb/204Pb = 18.325-18.362, 207Pb/204Pb = 15.523-5.562, 208Pb/204Pb = 38.064-38.221) revealed that ore metals primarily originated from magma. Based on this research, the origin of the Batailing Au deposit is of the mesothermal magmatic-hydrothermal lode type.