Genetically encoded epigenetic sensors for visualization of H3K9me3, H3K9ac and H3K4me1 histone modifications in living cells

被引：0
|
作者：
Stepanov, Afanasii I. [1 ,2 ]
Putlyaeva, Lidia, V [1 ,2 ]
Besedovskaya, Zlata [1 ]
Shuvaeva, Alexandra A. [1 ,2 ,3 ]
Karpenko, Nikita, V [2 ,4 ]
Rukh, Shah [1 ]
Gorbachev, Dmitry A. [2 ]
Malyshevskaia, Kseniia K. [2 ]
Terskikh, Alexey, V [5 ]
Lukyanov, Konstantin A. [2 ]
Gurskaya, Nadya G. [1 ,2 ,6 ]
机构：
[1] Skolkovo Inst Sci & Technol, Ctr Mol & Cellular Biol, Bolshoi Blvd 30,Bld 1, Moscow 121205, Russia
[2] Shemyakin Ovchinnikov Inst Bioorgan Chem, Miklukho Maklaya 16-10, Moscow 117997, Russia
[3] Moscow Inst Phys & Technol, Dolgoprudnyi 141701, Russia
[4] MIREA Russian Technol Univ, Lomonosov Inst Fine Chem Technol, Vernadskogo Pr 78, Moscow 119454, Russia
[5] Scintillon Res Inst, 6404 Nancy Ridge Dr, San Diego, CA 92121 USA
[6] Pirogov Russian Natl Res Med Univ, Ostrovityanova 1, Moscow 117997, Russia
来源：
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS | 2024年 / 733卷
基金：
俄罗斯科学基金会;
关键词：
Epigenetics; Histone modification; Fluorescent proteins; Genetically encoded sensor; H3K9me3; H3K9ac; H3K4me1; ACETYLATION; CHROMATIN;
D O I：
10.1016/j.bbrc.2024.150715
中图分类号：
Q5 [生物化学]; Q7 [分子生物学];
学科分类号：
071010 ; 081704 ;
摘要：
Post-translational modifications of histones play a crucial role in chromatin structure maintenance and epigenetic regulation. The LiveMIEL (Live-cell Microscopic Imaging of Epigenetic Landscape) method represents a promising approach for tracking histone modifications. It involves visualization of epigenetic modifications using genetically encoded fluorescent sensors and further analysis of the obtained intranuclear patterns by multi- parametric image analysis. In this study, we designed three new red fluorescent sensors-MPP8-Red, AF9-Red and DPF3-Red-for live-cell visualization of patterns of H3K9me3, H3K8ac and H3K4me1, respectively. The observed fluorescent patterns were visually distinguishable, and LiveMIEL analysis clearly classified them into three corresponding groups. We propose that these sensors can be used for live-cell dynamic analysis of changes in organization of three epigenetic types of chromatin.
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页数：7
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