Quaternized Poly(4-vinylpyridine-co-N-2-hydroxypropylmethacrylamide) and antibacterial peptide linear conjugates: Synthesis, characterization, and bioactivity
Cationic polymers and antibacterial peptides are effective in combating infections caused by bacteria. Although both materials can naturally exhibit antibacterial effects due to the disruption of the bacterial membrane, their combination can further enhance their properties. For instance, polymer-peptide conjugates can reduce the toxicity that cationic polymers exhibit against mammalian cells. In this study, poly(4-vinylpyridine-co-N-2hydroxypropylmethacrylamide) copolymer was RAFT-synthesized and conjugated to the antibacterial peptide of RWWRWWR through their chain ends via maleimide-thiol reaction. Subsequently, the pyridine groups of the produced linear conjugates were quaternized to obtain a novel conjugate of the antibacterial polycation and the antibacterial peptide. The structural characterization results acquired from FTIR, NMR, fluorescence spectroscopy and GPC revealed the successful synthesis of both the copolymer and the conjugate. The biological activity of the conjugate was evaluated on both bacteria and mammalian cells. The results indicated that reduced toxicity was achieved in the case of the conjugate compared to the copolymer, while the antibacterial activity of the conjugate against both E. coli and S. aureus was still maintained.
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Ningbo Univ, Fac Mat Sci & Chem Engn, Ningbo 315211, Zhejiang, Peoples R ChinaNingbo Univ, Fac Mat Sci & Chem Engn, Ningbo 315211, Zhejiang, Peoples R China
Lu, Linchao
Wang, Wenqin
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Ningbo Univ, Fac Mat Sci & Chem Engn, Ningbo 315211, Zhejiang, Peoples R ChinaNingbo Univ, Fac Mat Sci & Chem Engn, Ningbo 315211, Zhejiang, Peoples R China
Wang, Wenqin
Cai, Wujin
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Ningbo Univ, Fac Mat Sci & Chem Engn, Ningbo 315211, Zhejiang, Peoples R ChinaNingbo Univ, Fac Mat Sci & Chem Engn, Ningbo 315211, Zhejiang, Peoples R China
Cai, Wujin
Chen, Zhong-Ren
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Ningbo Univ, Fac Mat Sci & Chem Engn, Ningbo 315211, Zhejiang, Peoples R ChinaNingbo Univ, Fac Mat Sci & Chem Engn, Ningbo 315211, Zhejiang, Peoples R China
机构:Beijing University of Chemical Technology,State Key Laboratory of Chemical Resource Engineering, Beijing Advanced Innovation Center for Soft Matter Science and Engineering, Beijing Laboratory of Biomedical Materials
Jin-Rui Deng
Cong-Lei Zhao
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机构:Beijing University of Chemical Technology,State Key Laboratory of Chemical Resource Engineering, Beijing Advanced Innovation Center for Soft Matter Science and Engineering, Beijing Laboratory of Biomedical Materials
Cong-Lei Zhao
Yi-Xian Wu
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机构:Beijing University of Chemical Technology,State Key Laboratory of Chemical Resource Engineering, Beijing Advanced Innovation Center for Soft Matter Science and Engineering, Beijing Laboratory of Biomedical Materials
Yi-Xian Wu
Chinese Journal of Polymer Science,
2020,
38
: 704
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714
机构:
Inst Sci Tokyo, Sch Mat & Chem Technol, Dept Mat Sci & Engn, 2-12-1-S8-36 Ookayama,Meguro Ku, Tokyo 1528552, JapanInst Sci Tokyo, Sch Mat & Chem Technol, Dept Mat Sci & Engn, 2-12-1-S8-36 Ookayama,Meguro Ku, Tokyo 1528552, Japan
Kong, Youngwon
Kawahara, Hitomi
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Inst Sci Tokyo, Sch Mat & Chem Technol, Dept Mat Sci & Engn, 2-12-1-S8-36 Ookayama,Meguro Ku, Tokyo 1528552, JapanInst Sci Tokyo, Sch Mat & Chem Technol, Dept Mat Sci & Engn, 2-12-1-S8-36 Ookayama,Meguro Ku, Tokyo 1528552, Japan
Kawahara, Hitomi
Kikuchi, Ryohei
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Inst Sci Tokyo, Core Facil Ctr, Res Infrastructure Management Ctr, Mat Anal Div, 2-12-1 S7-26 Ookayama,Meguro Ku, Tokyo 1528552, JapanInst Sci Tokyo, Sch Mat & Chem Technol, Dept Mat Sci & Engn, 2-12-1-S8-36 Ookayama,Meguro Ku, Tokyo 1528552, Japan
Kikuchi, Ryohei
Ohta, Noboru
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Japan Synchrotron Radiat Res Inst, 1-1-1 Kouto, Sayo, Hyogo 6795198, JapanInst Sci Tokyo, Sch Mat & Chem Technol, Dept Mat Sci & Engn, 2-12-1-S8-36 Ookayama,Meguro Ku, Tokyo 1528552, Japan
Ohta, Noboru
Mufundirwa, Albert
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Japan Synchrotron Radiat Res Inst, 1-1-1 Kouto, Sayo, Hyogo 6795198, JapanInst Sci Tokyo, Sch Mat & Chem Technol, Dept Mat Sci & Engn, 2-12-1-S8-36 Ookayama,Meguro Ku, Tokyo 1528552, Japan
Mufundirwa, Albert
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Hirai, Tomoyasu
Hatakeyama-Sato, Kan
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Inst Sci Tokyo, Sch Mat & Chem Technol, Dept Mat Sci & Engn, 2-12-1-S8-36 Ookayama,Meguro Ku, Tokyo 1528552, JapanInst Sci Tokyo, Sch Mat & Chem Technol, Dept Mat Sci & Engn, 2-12-1-S8-36 Ookayama,Meguro Ku, Tokyo 1528552, Japan
Hatakeyama-Sato, Kan
Nabae, Yuta
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Inst Sci Tokyo, Sch Mat & Chem Technol, Dept Mat Sci & Engn, 2-12-1-S8-36 Ookayama,Meguro Ku, Tokyo 1528552, JapanInst Sci Tokyo, Sch Mat & Chem Technol, Dept Mat Sci & Engn, 2-12-1-S8-36 Ookayama,Meguro Ku, Tokyo 1528552, Japan
Nabae, Yuta
Hayakawa, Teruaki
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Inst Sci Tokyo, Sch Mat & Chem Technol, Dept Mat Sci & Engn, 2-12-1-S8-36 Ookayama,Meguro Ku, Tokyo 1528552, JapanInst Sci Tokyo, Sch Mat & Chem Technol, Dept Mat Sci & Engn, 2-12-1-S8-36 Ookayama,Meguro Ku, Tokyo 1528552, Japan