Expression of Recombinant Human α-Glucosidase in HEK293 Cells

被引：0

作者：

Nishimoto, So ^{[1
]}

Debarbat, Anais ^{[1
]}

Ikeda, Yuki ^{[1
]}

Arikawa, Emi ^{[1
]}

Odagaki, Yuki ^{[1
]}

Yano, Haruna ^{[1
]}

Qiao, Ying ^{[1
]}

Ito, Masaaki ^{[2
]}

Kimura, Toshiyuki ^{[3
]}

Takita, Teisuke ^{[1
]}

Yasukawa, Kiyoshi ^{[1
]}

机构：

[1] Kyoto Univ, Grad Sch Agr, Div Food Sci & Biotechnol, Sakyo Ku, Kyoto 6068502, Japan

[2] Natl Inst Technol, Okinawa Coll, Dept Bioresources Engn, Henoko, Nago 9052192, Japan

[3] Natl Agr & Food Res Org NARO, Res Ctr Adv Anal, Tsukuba 3058642, Japan

来源：

JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY | 2024年 / 73卷 / 01期

基金：

日本学术振兴会;

关键词：

alpha-glucosidase; HEK293; maltase-glucoamylasecomplex; sucrase-isomaltase complex; INTESTINAL MALTASE-GLUCOAMYLASE; SUCRASE-ISOMALTASE; CACO-2; CELLS; MORUS-ALBA; INHIBITION; LEAVES; SUGARS;

D O I：

10.1021/acs.jafc.4c06902

中图分类号：

S [农业科学];

学科分类号：

09 ;

摘要：

In mammals, intestinal alpha-glucosidase exists as a maltase-glucoamylase complex (MGAM) and a sucrase-isomaltase complex (SI). In this study, we transiently expressed human MGAM and SI in human embryonic kidney 293 (HEK293) cells. At pH 6.0 and 37 degrees C, the MGAM-expressing HEK293 cells extract (MGE) exhibited maltase, glucoamylase, and isomaltase activities but not sucrase activity, whereas the SI-expressing HEK293 cells extract (SIE) exhibited sucrase, isomaltase, and maltase activities but not glucoamylase activity. The apparent K m value of the MGE for maltose hydrolysis was 14-26% of that of the SIE for maltose, sucrose, and isomaltose hydrolysis. The respective apparent V max values of the MGE and SIE for sucrose and isomaltose hydrolysis were 0% and 6% and 10% and 42% of those for maltose hydrolysis. These results indicated that the maltase activities of MGAM and SI were higher than those of sucrase and isomaltase.

引用

页码：617 / 624

页数：8

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