EVALUATION OF NEUTROPHIL EXTRACELLULAR TRAP FORMATION ON SMOKERS AND NON-SMOKERS WITH PERIODONTITIS

Objective: It's aimed to evaluate neutrophil extracellular trap (NET) in smoker and non-smoker periodontitis patients. Materials and Methods: 21 subjects were included in this study and divided into three groups [nonsmokers and periodontally healthy (S-P-), non-smokers with periodontitis (S-P+), smokers with periodontitis (S+P+)]. Clinical attachment level (CAL), probing depth (PD), plaque index (PI), and gingival index (GI) parameters were recorded. To induce NET formation the peripheral neutrophils were isolated from the subjects and were activated by phorbol 12-myristate 13-acetate (PMA). Active NET was measured with the fluorometer. Results: PI, GI, PD and CAL values were found statistically significant low in the S-P- group (P=0.001; P=<0.001; P=0.003; P=<0.001, respectively). NET count values were the highest in the S+P+ group. There was statistically significant difference between S-P- group and S+P+ group was (P=0.003); it was not statistically significant between S-P+ and S+P+ groups (P>0.05). NET count with CAL has strong positive relation and with PI, GI, PD, has mild positive relation. Conclusion: As a result, there was no statistically significant difference between S-P+ and S+P+ groups, even the S+P+ group NET count was found higher. Further researches are needed on the effect smoking on NET formation in patients with periodontitis.