Protocol to measure glucose utilization in mouse tissues using radiolabeled 2-deoxy-D-glucose

被引：0

作者：

Wu, Cyun-Ming ^{[1
]}

Li, Hao-Yun ^{[1
]}

Li, Wen-Yi ^{[2
,4
]}

Hsu, Chih-Neng ^{[3
]}

Yang, Wei-Shun

Chuang, Gwo-Tsang ^{[5
]}

Che, Tony Pan-Hou ^{[6
,7
]}

Lee, Tung-Yuan ^{[6
,7
]}

Lee, Hsiao-Lin ^{[1
,14
]}

Hee, Siow-Wey ^{[8
]}

Lee, Jiin-Horng ^{[14
]}

Liao, Daniel ^{[8
]}

Liao, Karen Chia-Wen ^{[9
]}

Liu, Ya-Wen ^{[10
]}

Hsuan, Chin-Feng ^{[11
,12
,13
,14
]}

Chang, Yi-Cheng ^{[6
,7
,8
,14
,15
]}

Chu, Shao-Lun ^{[1
]}

机构：

[1] Natl Taiwan Univ, Coll Med, Dept Med Genet, Taipei 100, Taiwan

[2] Natl Taiwan Univ Hosp, Yunlin Branch, Dept Internal Med, Nephrol Div, Yunlin, Taiwan

[3] Natl Taiwan Univ Hosp, Cardiovasc Ctr, Yunlin Branch, 579,Sec 2,Yunlin Rd, Yunlin 640, Taiwan

[4] Natl Taiwan Univ Hosp, Hsin Chu Branch, Dept Internal Med, Div Nephrol, Hsinchu 300, Taiwan

[5] Natl Taiwan Univ Hosp, Dept Pediat, Div Pediat Nephrol, Taipei 100, Taiwan

[6] Natl Taiwan Univ, PhD Program Translat Med, Taipei 100, Taiwan

[7] Acad Sinica, Taipei 100, Taiwan

[8] Natl Taiwan Univ Hosp, Div Endocrinol & Metab, Dept Internal Med, Taipei 100, Taiwan

[9] Univ Chicago, Div Biol Sci, Chicago, IL 60637 USA

[10] Natl Taiwan Univ, Grad Inst Mol Med, Taipei 100, Taiwan

[11] I Shou Univ, E Da Hosp, Dept Internal Med, Div Cardiol, Kaohsiung 824, Taiwan

[12] I Shou Univ, E Da Dachang Hosp, Dept Internal Med, Div Cardiol, Kaohsiung, Taiwan

[13] I Shou Univ, Coll Med, Sch Med, Kaohsiung 824, Taiwan

[14] Natl Taiwan Univ, Grad Inst Med Genom & Prote, Taipei 100, Taiwan

[15] Acad Sinica, Inst Biomed Sci, Taipei, Taiwan

来源：

STAR PROTOCOLS | 2024年 / 5卷 / 04期

关键词：

MOLECULAR-MECHANISMS; SKELETAL-MUSCLE; ADIPOSE-TISSUE;

D O I：

10.1016/j.xpro.2024.103478

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

2-deoxy-D-glucose (2DG) is a glucose analog converted to 2-deoxy-D-glucose-6phosphate (2DG-6P) by hexokinase in glycolysis. While 2DG commonly measures glucose uptake, 2DG-6P detects glucose utilization. Here, we present a protocol to measure glucose utilization in various tissues after entering a mouse's body using radiolabeled 2DG. We describe steps for preparing mice and chemicals, extracting blood, adding chemicals, and dissolving tissue. We then detail procedures for calculating glucose utilization using the trapezoid rule.

引用

页数：16

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