Unveiling the impact of simulated microgravity on HSV-1 infection, neuroinflammation, and endogenous retroviral activation in SH-SY5Y cells

被引:0
|
作者
Jasemi, Seyedesomaye [1 ]
Simula, Elena Rita [1 ]
Yasushi, Kawaguchi [2 ,3 ]
Sechi, Leonardo Antonio [1 ,4 ]
机构
[1] Univ Sassari, Dept Biomed Sci, Div Microbiol & Virol, Sassari, Italy
[2] Univ Tokyo, Div Mol Virol, Inst Med Sci, Dept Microbiol & Immunol, Minato Ku, Tokyo 1088639, Japan
[3] Univ Tokyo, Inst Med Sci, Dept Infect Dis Control, Int Res Ctr Infect Dis, Minato Ku, Tokyo 1088639, Japan
[4] Azienda Osped Univ Sassari, Struttura Complessa Microbiol & Virol, Sassari, Italy
关键词
Microgravity; HSV-1; Herpes simplex virus 1; HERV; Immune response; SIMPLEX-VIRUS TYPE-1; HERPESVIRUS; REPLICATION; EXPRESSION; LATENT;
D O I
10.1007/s13365-025-01251-0
中图分类号
Q189 [神经科学];
学科分类号
071006 ;
摘要
Microgravity (mu g) during spaceflight affects cellular and molecular functions of both human cells and microbial pathogens, influencing viral replication and the host immune system. This study aimed to investigate the effects of simulated mu g on Herpes Simplex Virus-1 (HSV-1) replication, host pro-inflammatory cytokine, and human endogenous retrovirus (HERV) activation in human neuroblastoma SH-SY5Y cells. Our results show that mu g has a negative impact on HSV-1 replication, leading to significantly reduced viral titers and lower expression levels of HSV-1 early genes (ICP0, ICP4, and ICP27) compared to 1 gravity (1 g) conditions. Interestingly, despite lower viral titers and HSV-1 gene expressions under mu g condition, we observed higher levels of HERVs and pro-inflammatory cytokine gene expression. In addition, there was a significant correlation between HSV-1 immediate-early genes with HERVs and pro-inflammatory cytokine gene expression, with stronger correlations observed under mu g conditions. Taken together, mu g reduces HSV-1 replication and increases host pro-inflammatory and HERVs gene expression, which demands further investigation for human health protection in space.
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页数:9
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