Chromosome-level Haploid Assembly of Cannabis sativa L. cv. Pink Pepper

As molecular research on hemp (Cannabis sativa L.) continues to advance, there is a growing need for the accumulation of more diverse genome data and more accurate genome assemblies. In this study, we report the three-way assembly data of a cannabidiol (CBD)-rich cannabis variety, ‘Pink Pepper’ cultivar using sequencing technology: PacBio Single Molecule Real-Time (SMRT) technology, Illumina sequencing technology, and Oxford Nanopore Technology (ONT). This assembly anchors scaffolds to the ten chromosomes of hemp, and to avoid confusion with previous cannabis genetic research, the chromosomes have been labeled based on an earlier reference genome. The total assembled genome length is 770 Gbp, with a GC content of 34.09% and a repeat region accounting for 77.13% of the genome. This assembly, which incorporates the unique strengths of the three sequencing technologies, demonstrated the highest complete BUSCO scores (97.8%-99.6%) among the reported cannabis genomes, as evaluated using three different BUSCO databases. With annotations for 30,459 protein-coding genes, this dataset can serve as a valuable resource for advancing genetic research on hemp.