In vitro and in vivo assessment of a new acellular human amnion/chorion membrane device for guided bone regeneration

被引:0
|
作者
Galvez, Paul [1 ,2 ]
Omar, Naima Ahmed [1 ]
Siadous, Robin [1 ]
Durand, Marlene [1 ,3 ,4 ]
Comperat, Leo [1 ]
Lafarge, Xavier [5 ,6 ]
Gindraux, Florelle [7 ,8 ]
Sentilhes, Loic [9 ]
Fricain, Jean-Christophe [1 ,2 ,10 ]
L'Heureux, Nicolas [1 ]
Fenelon, Mathilde [1 ,2 ,10 ]
机构
[1] Univ Bordeaux, Biotis, INSERM, U1026, 146 Rue Leo Saignat, F-33000 Bordeaux, France
[2] CHU Bordeaux, Serv Chirurg Orale, Pl Amelie Raba Leon, F-33076 Bordeaux, France
[3] CHU Bordeaux, CIC 1401, Pl Amelie Raba Leon, F-33000 Bordeaux, France
[4] Univ Bordeaux, Inst Bergonie, INSERM, CIC 1401, 146 Rue Leo Saignat, F-33000 Bordeaux, France
[5] Etab Francais Sang Nouvelle Aquitaine, Lab ingenierie Tissulaire & Cellulaire, Pl Amelie Raba Leon, F-33000 Bordeaux, Aquitaine, France
[6] Univ Bordeaux, INSERM, U1211, Maladies Rares Genet & Metab, 146 Rue Leo Saignat, F-33000 Bordeaux, France
[7] CHU Besancon, Serv Chirurg Maxillo Faciale, Stomatol & Odontol Hosp, 3 Blvd Alexandre Fleming, F-25000 Besancon, France
[8] Univ Marie & Louis Pasteur, SINERGIES, 16 Route Gray, F-25000 Besancon, France
[9] CHU Bordeaux, Serv Gynecol Obstet, Pl Amelie Raba Leon, F-33076 Bordeaux, France
[10] Ctr Competence Malad Rares Orales & Dentaires, CHU Bordeaux, O RARES, Pole Odontol & Sante Buccale, Pl Amelie Raba Leon, F-33076 Bordeaux, France
来源
SCIENTIFIC REPORTS | 2025年 / 15卷 / 01期
关键词
Amnion-chorion; Amniochorionic membrane; Placental membranes; Guided bone regeneration; In vivo; Acellular scaffold; Decellularization; HUMAN AMNIOTIC MEMBRANE; HORIZONTAL RIDGE AUGMENTATION; HUMAN FETAL MEMBRANES; EXTRACELLULAR-MATRIX; CHORION MEMBRANES; CASE SERIES; RESORBABLE MEMBRANES; TISSUE REGENERATION; INTRABONY DEFECTS; IMPLANT PLACEMENT;
D O I
10.1038/s41598-025-88814-7
中图分类号
O [数理科学和化学]; P [天文学、地球科学]; Q [生物科学]; N [自然科学总论];
学科分类号
07 ; 0710 ; 09 ;
摘要
Thanks to its unique biological properties, the human amniotic membrane (AM) has shown promising results for guided bone regeneration (GBR), but displays some limitations such as poor space-maintaining ability. This study thus aimed to develop a new amnion/chorion membrane (ACM), with better mechanical properties as well as comparable or improved biological properties for GBR. We first developed a new decellularization method of ACM (DL-ACM) which was validated by DNA staining and quantification, and its cytocompatibility was established in vitro. The thickness of DL-ACM was significantly increased over thirty-fivefold, and its tearing strength and compression strength significantly increased more than tenfold compared to the decellularized AM (DL-AM). In vivo, DL-ACM demonstrated its biocompatibility subcutaneously, and its osteogenic properties were compared to DL-AM and a gold standard membrane in a GBR defect model in rats. Micro-CT and histomorphometric analysis showed that DL-ACM significantly promoted early bone regeneration after 1 week and significantly increased bone regeneration compared to the empty defect and the gold standard membrane over time. In this study, we developed a simple and reproducible method to produce an acellular, non-cytotoxic, and biocompatible DL-ACM. This new membrane is as effective as AM to promote early bone regeneration while demonstrating better biomechanical properties.
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页数:22
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