Two-dimensional materials represented by graphene possess excellent comprehensive performance, which can be used to manufacture functional nano-inks and obtain optoelectronic functional devices by ink-jet printing. There are broad application prospects in the fields of energy, environment, life and health. This paper systematically summarized the properties of several typical two-dimensional materials, the preparation of nano-inks and the application of ink-jet printing, focusing on the regulation of liquid phase exfoliation and ink-jet printing processes. Different exfoliation methods and principles were summarized and compared, as well as the related theories of ink-jet printing, such as the coffee ring effect and Marangoni effect, were reviewed. Through the precise regulation of the two-dimensional material exfoliation process and its nano-inks printing process, it was expected to form an advanced micro-nano manufacturing technology based on ink-jet printing, realize the low-cost processing of different functional micro-nano electronic components and devices, and promote the development of biosensing, flexible electronics and other fields. © 2024 Chemical Industry Press Co., Ltd.. All rights reserved.