Mechanism of Cr(VI) bioreduction by Clostridium sp. LQ25 under Fe(III) reducing conditions

The Cr(VI) bioreduction has attracted widespread attention in the field of Cr(VI) pollution remediation due to its environmental friendliness. Further in-depth research on the reduction mechanisms is necessary to enhance the efficiency of Cr(VI) bioreduction. However, the limited research on Cr(VI) bioreduction mechanisms remains a bottleneck for the practical application of Cr(VI) reduction. In this study, The Cr(VI) reduction of strain LQ25 was significantly improved when Fe(III) was used as an electron acceptor, which increased by 1.6-fold maximum within the set Cr(VI) concentration range. Based on this, the electron transfer process of Cr(VI) reduction was analyzed using strain LQ25. Based on genomic data, flavin proteins were found to interact closely with electron transfer-related proteins using protein–protein interaction (PPi) analysis. Transcriptome analysis revealed that flavin synthesis genes (ribE, ribBA, and ribH) and electron transfer flavoprotein genes (fixA, etfA, fixB, and etfB) were significantly upregulated when Fe(III) was used as the electron acceptor. These results indicate that the fermentative dissimilatory Fe(III)-reducing bacterial strain LQ25 mainly uses flavin as an electron shuttle for electron transfer, which differs from the common use of cytochrome c in respiratory bacteria. These findings on the mechanism of Cr(VI) bioreduction provide technical support for improving the efficiency of Cr(VI) reduction which promote the practical application of Cr(VI) bioreduction in the field of Cr(VI) pollution remediation. © 2024 Elsevier Ltd