PRODUCTION OF MICROBIAL ENZYMES AND THEIR APPLICATIONS TO CLINICAL ANALYSIS.

This article reviews recent developments in use of microbial enzymes for clinical analysis and new trends in production of the enzymes by use of genetic engineering techniques. The increased availability of microbial enzymes has led to many analytical applications in the areas of clinical chemistry. Especially during the last decade enzymatic assays have rapidly spread in clinical laboratories through efforts of the search of useful enzymes. The applications of cholesterol oxidase to the determination of serum cholesterol showed that the enzymatic method was more specific, precise and sensitive than the chemical method. This success stimulated developments and adoptions of other enzymatic assays for triglycerides, phospholipids and free fatty acids.