Cloning and expression of the rabbit growth hormone cDNA

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作者：
Dong, Yuqing
Li, Ronggui
Zhao, Jindong
Wu, Guangyao
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Beijing Daxue Xuebao Ziran Kexue Ban/Acta Scientiarum uaturalium Universitatis Pekinensis | 2000年 / 36卷 / 02期
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摘要：
The cDNA of preprotein and mature protein of rabbit hypophysiscerebri was obtained. The cDNA of mature growth hormone was cloned in pET-3a, then the recombinant expression vector was transformed into E. coli BL21 (DE3 plys). The overexpression was performed and the yield was 40% of the total protein.
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页码：199 / 202
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