Construction and nucleotide sequence assay of human anti-HBs variable region single-chain antibody gene

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作者：
Ren, Xiangrong
Gao, Hui
Su, Kuanyuan
Chen, Wenyin
Yang, Angang
Yu, Zhouyao
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Shengwu Yixue Gongchengxue Zazhi/Journal of Biomedical Engineering | 2000年 / 17卷 / 04期
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摘要：
The VH and Vk gene which were acquired using phage display technology have been combined by a linker encoding a flexible peptide (Gly4 Ser)3 to form a single-chain. Its nucleotide sequence was assayed after cloning the scFv gene into middle plasmid. The successful construction of single-chain gene lays the foundations for the subsequent expression and production of double functional antibody.
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页码：484 / 486
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