Effect of calmodulin and voltage-dependent Ca2+ channel on the proliferation of heptoma cells induced by epidermal growth factor

被引：0

作者：

Wu, Binwen ^{[1
]}

Wang, Jialong ^{[1
]}

Yuan, Shunyu ^{[1
]}

Cui, Wuren ^{[2
]}

机构：

[1] Institute of Liver Disease, Tongji Hospital, Huazhong University of Science and Technology, Wuhan 430030

[2] Department of Nuclear Medicine, Tongji Medical College, Huazhong University of Science and Technology, Wuhan 430030

来源：

Journal of Huazhong University of Science and Technology - Medical Science | 2003年 / 23卷 / 01期

关键词：

Biosynthesis - Cells - DNA - Electric potential - Enzyme kinetics;

D O I：

暂无

中图分类号：

学科分类号：

摘要：

The effect of thyrosine kinase, calmodulin and voltage-dependent Ca 2+ channel on the proliferation of hepatoma cells induced by EGF was studied. Hepatoma cell line SMMC7721 was cultured in RPMI1640 serum-free medium. DNA synthesis rate of hepatoma cells was measured byH-TdR incorporation. 10-9 mol/L EGF could significantly stimulate the proliferation of hepatoma cells (P 0.05), but it had very significantly inhibitory effect on the proliferation of hepatoma cells induced by EGF (P2+ channel inhibitor Varapamil had no inhibition on the proliferation of hepatoma cells induced by EGF (P>0.05). It had no effect on the basic phase of cultured hepatoma cells (P>0.05). It is suggested that tyrosine kinase and Ca2+- calmodulin-dependent pathway may play a critical role on the proliferation of heptoma cells induced by EGF, and voltage-dependent Ca2+ channel is independent of the effect of EGF.

引用

页码：26 / 28

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