The Ramachandran plot and the NMR method for protein structure determination

The Ramachandran plot for visualization of the sterically allowable combinations of polypeptide backbone dihedral angles i and ψi in the - ψ plane has had considerable impact on the development of the now widely used NMR method for protein structure determination in solution. This paper first presents a brief survey of the determination of NMR structures of proteins. It then focuses on the role played by the Ramachandran plot in the development of a general strategy for sequential NMR assignments, and the identification of regular secondary polypeptide structures by NMR. © (1990), (Indian Academy of Sciences). All Rights Reserved.