Detection of bovine meat and bone meal in animal feed at a level of 0.1%

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作者
Aarts, Henk J. M. [1 ]
Bouw, El M. [1 ]
Buntjer, Jaap B. [2 ]
Lenstra, Johannes A. [3 ]
Van Raamsdonk, Leo W. D. [1 ]
机构
[1] RIKILT-Institute of Food Safety, Wageningen UR, Bornsesteeg 45, 6708 PD Wageningen, Netherlands
[2] Keygene N.V., PO Box 216, 6700 AE Wageningen, Netherlands
[3] Utrecht University, Faculty of Veterinary Medicine, Yalelaan 8, 3584 CM Utrecht, Netherlands
来源
Journal of AOAC International | 1600年 / 89卷 / 06期
关键词
For the control of the transmission of bovine spongiform encephalopathy in cattle via feedstuff; a real-time polymerase chain reaction assay was developed with ruminant-specific Bov-B SINE primers; SYBR® Green fluorescence detection; and melting curve analysis. In formulated cattle and chicken feed samples spiked with pure bovine and sheep meat and bone meal heated at 133°C for 20 min; a contamination level of 0.1% was detected;
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页码:1443 / 1446
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