Microchip capillary electrophoresis-mass spectrometry for high-throughput simultaneous analysis of B-complex vitamins

被引：0
|
作者：
Yang, Liu [1 ]
Wang, Shuang [3 ]
Li, Xiaolong [1 ]
Wang, Weimin [1 ,2 ]
Xu, Fuxing [1 ,2 ]
Ding, Chuan-Fan [1 ,2 ]
机构：
[1] School of Material Science and Chemical Engineering, Ningbo University, Ningbo,315211, China
[2] Key Laboratory of Advanced Mass spectrometry and Molecular Analysis of Zhejiang Province, Ningbo University, Ningbo,315211, China
[3] Zheng Zhou Tobacco Research Institute of CNTC, Zheng Zhou,450001, China
来源：
Journal of Chromatography A | 2025年 / 1740卷
基金：
中国国家自然科学基金;
关键词：
Capillarity;
D O I：
10.1016/j.chroma.2024.465589
中图分类号：
学科分类号：
摘要：
B-complex vitamins are essential micronutrients that maintaining health, and provide (individually/simultaneously) many important biological actions in organism. Therefore, sensitive, reliable analytical method to determine B-complex vitamins simultaneously in actual samples is significant. Conventional analytical methods for vitamins analysis are usually labor-intensive, time-consuming and mostly do not allow the simultaneous determination. Few papers have been published on capillary electrophoresis-mass spectrometry (CE-MS) analysis of individual vitamin B or B-complex vitamins. Microchip capillary electrophoresis (ME) offers less consumption of background electrolyte and reagents than CE, and fast separation as having several channels for high-throughput. ME based on ZipChip integrates sample injection, electrophoretic separation, and ESI functions into one platform, herein, for the first time, in this paper, the ME-MS based on ZipChip platform was investigated and demonstrated preliminarily a significant improvement over existing techniques. Because: the ME-MS method has a linear response with excellent precision for replicate injections and LODs lower than 4 ppb; The method is simple and fast ( © 2024 Elsevier B.V.
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