Molecular disruptions in microalgae caused by Acidithiobacillus ferrooxidans: Photosynthesis, oxidative stress, and energy metabolism in acid mine drainage

Microalgae are recognized for their potential in the bioremediation of acid mine drainage (AMD), despite the challenges posed by AMD's low pH, high heavy metal content, and oligotrophic conditions. However, the impact of AMD chemoautotrophic microorganisms on microalgal growth and remediation efforts within AMD has been largely overlooked. This study aims to elucidate the effects the chemoautotrophic microorganism, Acidithiobacillus ferrooxidans, on the growth activity and metabolism of acid-tolerant microalgae, and to explore the molecular mechanisms of microalgal response. Our findings reveal that the presence of A. ferrooxidans inhibits the growth and alkaline production of Parachlorella sp. MP1, resulting in a 90.86 % reduction in biomass. Physiological, biochemical, and transcriptomic studies, indicate that oxidative stress, photosynthesis, and energy metabolism are the metabolic processes most affected by A. ferrooxidans. Specifically, A. ferrooxidans introduces an increased production of reactive oxygen species (ROS) in Parachlorella sp. MP1, leading to an upregulation of genes and enzymes associated with peroxisome activity and intensifying oxidative stress within the cells. Downregulation of photosynthesis-related genes disrupts the electron transport chain, inhibiting photosynthesis. Furthermore, alterations in the gene expression of pyruvate and acetyl-CoA metabolic pathways result in energetic pathway disruption. These insights contribute to a better understanding of how A. ferrooxidans influence the growth metabolism of acid-tolerant microalgae in AMD environments and inform the optimization of microalgal application strategies in AMD bioremediation engineering.