Preparation and evaluation of monoclonal antibody against nitroaniline

被引：0
|
作者：
Department of Environmental Science and Engineering, Tsinghua University, Beijing 100084, China [1 ]
不详 [2 ]
机构：
来源：
Qinghua Daxue Xuebao | 2008年 / 12卷 / 2079-2082期
关键词：
Bovine serum albumins - ELISA screenings - Environmental Monitoring - Enzyme-linked immunosorbent assays - Hybridoma technology - Indirect competitive ELISA - Intraperitoneally - Nitroanilines;
D O I：
暂无
中图分类号：
学科分类号：
摘要：
A monoclonal antibody (McAb) against nitroaniline was prepared for measuring nitroaniline residuals and for an enzyme linked immuno-sorbent assay (ELISA) reagent kit. Balb/c mice were immunized intraperitoneally with nitroaniline coupled with bovine serum albumin. Hybridoma cell lines secreting McAbs were obtained using routine hybridoma technology and ELISA screening. The McAbs subtype was IgG1. The ascite titer was 10-7. The McAbs had no cross-reaction with some analogues of nitroaniline. The results show that the indirect competitive ELISA can be used to accurately detect nitroaniline residues in various water samples and be benefit for environmental monitoring.
引用
收藏
相关论文
共 50 条
  • [21] PREPARATION OF A MONOCLONAL-ANTIBODY AGAINST HUMAN MONOCYTE LINEAGE
    MARUYAMA, S
    NAITO, T
    KAKITA, H
    KISHIMOTO, S
    YAMAMURA, Y
    KISHIMOTO, T
    JOURNAL OF CLINICAL IMMUNOLOGY, 1983, 3 (01) : 57 - 64
  • [22] Preparation of monoclonal antibody against human coagulation factor VII
    Xiao, X.
    Xu, S.
    Xiao, L.
    Ling, F.
    Zhao, Q.
    Hou, Y.
    Ma, L.
    Wang, Z.
    VOX SANGUINIS, 2008, 95 : 235 - 235
  • [23] Preparation and Application of Monoclonal Antibody Against hNDRG2
    Xinping Liu
    Xiaolan Hu
    Jian Zhang
    Lifeng Wang
    Wenghong Zhang
    Xuesong Liu
    Fuyang Li
    Yuanqiang Zhang
    Libo Yao
    Applied Biochemistry and Biotechnology, 2009, 152 : 306 - 315
  • [24] PREPARATION AND CHARACTERIZATION OF A MONOCLONAL-ANTIBODY AGAINST BOAR ACROSIN
    PEKNICOVA, J
    CAPKOVA, J
    CECHOVA, D
    SULCOVA, B
    FOLIA BIOLOGICA, 1986, 32 (04) : 282 - 285
  • [25] Production & evaluation of a monoclonal antibody against human myeloperoxidase
    Badrinath, Y
    Dhond, SR
    Bajpai, S
    Nair, CN
    Narkar, AA
    Advani, SH
    INDIAN JOURNAL OF MEDICAL RESEARCH, 1997, 105 : 176 - 179
  • [26] PRODUCTION AND APPLICATION OF MONOCLONAL ANTIBODY TO POLYAMINE (PREPARATION AND CHARACTERISTICS OF MONOCLONAL ANTIBODIES AGAINST SPERMIDINE)
    王德斌
    陈智周
    范振符
    曹明华
    田京燕
    Chinese Journal of Cancer Research, 1991, (04) : 40 - 45
  • [27] Preparation and Evaluation of the Fully Humanized Monoclonal Antibody GD-mAb Against Respiratory Syncytial Virus
    Tian, Puyuan
    Wang, Yuqing
    Liu, Hui
    Yang, Yulu
    Wu, Xiaoli
    Wei, Hua
    Chen, Tingtao
    FRONTIERS IN CELLULAR AND INFECTION MICROBIOLOGY, 2019, 9
  • [28] Effective preparation of a monoclonal antibody against human chromogranin A for immunohistochemical diagnosis
    Zhang, Danping
    Xie, Chengjie
    Wang, Rongzhi
    Yang, Qinghai
    Chen, Huiling
    Ling, Sumei
    Wang, Shihua
    Jia, Kunzhi
    BMC BIOTECHNOLOGY, 2018, 18
  • [29] Preparation of a Monoclonal Antibody against Gintonin and Its Use in an Enzyme Immunoassay
    Lee, Byung-Hwan
    Choi, Sun-Hye
    Kim, Hyeon-Joong
    Jung, Seok-Won
    Kim, Hyunsook
    Shin, Ho-Chul
    Lee, Joon-Hee
    Hwang, Sung-Bee
    Kim, Hyoung-Chun
    Nah, Seung-Yeol
    BIOLOGICAL & PHARMACEUTICAL BULLETIN, 2015, 38 (10) : 1631 - 1637
  • [30] Preparation of monoclonal antibody against human m3 receptor
    Zeng, SJ
    Li, S
    Peng, DW
    Qiu, PX
    Wu, YP
    ACTA PHARMACOLOGICA SINICA, 2000, 21 (04): : 335 - 339
12345