Two distinct mutations in gyrA lead to ciprofloxacin and nalidixic acid resistance in Campylobacter coli and Campylobacter jejuni isolated from chickens and beef cattle

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作者：
Jesse, T.W. [1 ]
Englen, M.D. [1 ,2 ]
Pittenger-Alley, L.G. [1 ]
Fedorka-Cray, P.J. [1 ]
机构：
[1] Bacterial Epidemiology and Antimicrobial Resistance Research Unit, U.S. Department of Agriculture, Agricultural Research Service, Athens, GA, United States
[2] USDA, Bacterial Epidemiology and Antimicrobial Resistance Research Unit, Richard B. Russell Agricultural Research Center, 950 College Station Road, Athens, GA 30605-2720, United States
来源：
Journal of Applied Microbiology | 2006年 / 100卷 / 04期
关键词：
Aims: The aim of this study was to identify point mutations in the gyrA quinolone resistance determining region (QRDR) of Campylobacter coli (n = 27) and Campylobacter jejuni (n = 26) that confer nalidixic acid (NAL) resistance without conferring resistance to ciprofloxacin (CIP). Methods and Results: Point mutations in the QRDR of gyrA from C. coli and C. jejuni isolates were identified by direct sequencing. All isolates (n = 14) with minimum inhibitory concentrations (MICs) ≥4 μg ml-1 for CIP and ≥32 μg ml-1 for NAL possessed a missense mutation leading to substitution of Ile for Thr at codon 86. Three isolates with a missense mutation leading to a Thr86Ala substitution had MICs -1 for CIP and ≥32 μg ml-1 for NAL. Conclusions: These data confirm previous findings that Thr86Ile mutations confer resistance to both CIP and NAL. However; resistance to NAL alone was conferred by a single Thr86Ala mutation. Significance and Impact of the Study: Resistance to NAL alone arises independently from CIP resistance. In addition; the role of other previously described point mutations in quinolone resistance is discussed. © 2006 The Society for Applied Microbiology;
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页码：682 / 688
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