Analysis of Tear Glucose Concentration with Electrospray Ionization Mass Spectrometry

被引：0
|
作者：
Taormina, Christopher R. [1 ]
Baca, Justin T. [1 ]
Asher, Sanford A. [1 ]
Grabowski, Joseph J. [1 ]
Finegold, David N. [2 ]
机构：
[1] Department of Chemistry, University of Pittsburgh, Pittsburgh, PA, United States
[2] Department of Pediatrics, University of Pittsburgh School of Medicine, Pittsburgh, PA, United States
来源：
Journal of the American Society for Mass Spectrometry | 2007年 / 18卷 / 02期
关键词：
We have developed a mass spectrometry-based method that allows one to accurately determine the glucose concentration of tear fluid. We used a 1 μL micro-capillary to collect tear fluid from the tear meniscus with minimal irritation of the eye. We analyzed the 1 μL volume of collected tear fluid with liquid-chromatography electrospray ionization mass spectrometry with the use of D-glucose-6,6-d2 as an internal standard. Repeated measurements and a recovery experiment on pooled, onion-induced tears showed that the analysis of the glucose in tears was precise (4% relative standard deviation) and provided 100% recovery. We found the tear glucose concentration of one fasting nondiabetic subject to be 13 to 51 μM while the onion-induced tear glucose concentration of a different nondiabetic subject to be 211 to 256 μM. © 2007 American Society for Mass Spectrometry;
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页码：332 / 336
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