Improving nature's enzyme active site with genetically encoded unnatural amino acids

被引：0
|
作者：
Jackson, Jennifer C. [1 ]
Duffy, Sean P. [1 ]
Hess, Kenneth R. [1 ]
Mehl, Ryan A. [1 ]
机构：
[1] Franklin and Marshall College, Department of Chemistry, P.O. Box 3003, Lancaster, PA 17604, United States
来源：
Journal of the American Chemical Society | 2006年 / 128卷 / 34期
关键词：
The ability to site-specifically incorporate a diverse set of unnatural amino acids (>30) into proteins and quickly add new structures of interest has recently changed our approach to protein use and study. One important question yet unaddressed with unnatural amino acids (UAAs) is whether they can improve the activity of an enzyme beyond that available from the natural 20 amino acids. Herein; we report the >30-fold improvement of prodrug activator nitroreductase activity with an UAA over that of the native active site and a >2.3-fold improvement over the best possible natural amino acid. Because immense structural and electrostatic diversity at a single location can be sampled very quickly; UAAs can be implemented to improve enzyme active sites and tune a site to multiple substrates. © 2006 American Chemical Society;
D O I：
暂无
中图分类号：
学科分类号：
摘要：
Journal article (JA)
引用
收藏
页码：11124 / 11127
相关论文
共 50 条
  • [31] Recent advancements in enzyme engineering via site-specific incorporation of unnatural amino acids
    Hang-Qin Zhu
    Xiao-Ling Tang
    Ren-Chao Zheng
    Yu-Guo Zheng
    World Journal of Microbiology and Biotechnology, 2021, 37
  • [32] Recent advancements in enzyme engineering via site-specific incorporation of unnatural amino acids
    Zhu, Hang-Qin
    Tang, Xiao-Ling
    Zheng, Ren-Chao
    Zheng, Yu-Guo
    WORLD JOURNAL OF MICROBIOLOGY & BIOTECHNOLOGY, 2021, 37 (12):
  • [33] A Genetically Encoded Two-Dimensional Infrared Probe for Enzyme Active-Site Dynamics
    Wang, Li
    Zhang, Jia
    Han, Ming-Jie
    Zhang, Lu
    Chen, Chao
    Huang, Aiping
    Xie, Ruipei
    Wang, Guosheng
    Zhu, Jiangrui
    Wang, Yuchuan
    Liu, Xiaohong
    Zhuang, Wei
    Li, Yunliang
    Wang, Jiangyun
    ANGEWANDTE CHEMIE-INTERNATIONAL EDITION, 2021, 60 (20) : 11143 - 11147
  • [34] Probing Protein Folding Using Site-Specifically Encoded Unnatural Amino Acids as FRET Donors with Tryptophan
    Miyake-Stoner, Shigeki J.
    Miller, Andrew M.
    Hammill, Jared T.
    Peeler, Jennifer C.
    Hess, Kenneth R.
    Mehl, Ryan A.
    Brewer, Scott H.
    BIOCHEMISTRY, 2009, 48 (25) : 5953 - 5962
  • [35] Optical Control of a Neuronal Protein Using a Genetically Encoded Unnatural Amino Acid in Neurons
    Kang, Ji-Yong
    Kawaguchi, Daichi
    Wang, Lei
    JOVE-JOURNAL OF VISUALIZED EXPERIMENTS, 2016, (109):
  • [36] A genetically encoded multifunctional unnatural amino acid for versatile protein manipulations in living cells
    Ge Y.
    Fan X.
    Chen P.R.
    Chen, Peng R. (pengchen@pku.edu.cn), 1600, Royal Society of Chemistry (07): : 7055 - 7060
  • [37] Genetically encoded FRET sensors using a fluorescent unnatural amino acid as a FRET donor
    Ko, Wooseok
    Kim, Sanggil
    Lee, Seonghyun
    Jo, Kyubong
    Lee, Hyun Soo
    RSC ADVANCES, 2016, 6 (82): : 78661 - 78668
  • [38] A genetically encoded multifunctional unnatural amino acid for versatile protein manipulations in living cells
    Ge, Yun
    Fan, Xinyuan
    Chen, Peng R.
    CHEMICAL SCIENCE, 2016, 7 (12) : 7055 - 7060
  • [39] Mimicking Nature to Make Unnatural Amino Acids and Chiral Diamines
    So, Soon Mog
    Kim, Hyunwoo
    Mui, Leo
    Chin, Jik
    EUROPEAN JOURNAL OF ORGANIC CHEMISTRY, 2012, 2012 (02) : 229 - 241
  • [40] Enhancing Protein Stability with Genetically Encoded Noncanonical Amino Acids
    Li, Jack C.
    Liu, Tao
    Wang, Yan
    Mehta, Angad P.
    Schultz, Peter G.
    JOURNAL OF THE AMERICAN CHEMICAL SOCIETY, 2018, 140 (47) : 15997 - 16000
12345