Metal cation-induced deformation of DNA self-assembled monolayers on silicon: Vibrational sum frequency generation spectroscopy

被引：0
|
作者：
Asanuma, Hidehiko [1 ]
Noguchi, Hidenori [2 ]
Uosaki, Kohei [2 ]
Yu, Hua-Zhong [1 ]
机构：
[1] Department of Chemistry, Simon Fraser University, Burnaby, BC V5A 1S6, Canada
[2] Division of Chemistry, Graduate School of Science, Hokkaido University, Sapporo 060-0180, Japan
来源：
Journal of the American Chemical Society | 2008年 / 130卷 / 25期
关键词：
Nucleic acids possess charged phosphate groups in their backbones; which require counterions to reduce the repulsive Coulombic interactions between the strands. Herein we report how different mono-and divalent metal cations influence the molecular orientations of DNA molecules on silicon surfaces upon immobilization and hybridization. Our sum frequency generation (SFG) spectroscopy studies demonstrated that the degree of conformational variation of DNA self-assembled monolayers on silicon depends on the type of metal cations present. The molecular orientation change of immobilized single-stranded oligonucleotides correlates with DNA-cation affinity (Mg2+ > Ca2+ > K+ ∼ Na+): metal cations with the strongest affinity disrupt the structure of the underlying linker monolayer the most. Upon hybridization the trend is reversed; which is attributed to the greater ability of divalent cations to mask the negative charges on the DNA backbone. These findings provide useful information for the construction of more sensitive DNA biosensors; particularly the optimization of on-chip hybridization performance. © 2008 American Chemical Society;
D O I：
暂无
中图分类号：
学科分类号：
摘要：
Journal article (JA)
引用
收藏
页码：8016 / 8022
相关论文
共 50 条
  • [21] Self-assembled organic monolayers on gold nanoparticles: A study by sum-frequency generation combined with UV-vis spectroscopy
    Humbert, C
    Busson, B
    Abid, JP
    Six, C
    Girault, HH
    Tadjeddine, A
    ELECTROCHIMICA ACTA, 2005, 50 (15) : 3101 - 3110
  • [22] Vibrational spectroscopy of nitroaromatic self-assembled monolayers under extreme conditions
    Fu, Yuanxi
    Friedman, Elizabeth A.
    Brown, Kathryn E.
    Dlott, Dana D.
    CHEMICAL PHYSICS LETTERS, 2011, 501 (4-6) : 369 - 374
  • [23] Sum-frequency vibrational spectroscopic study of mixed alkylsiloxane monolayers self-assembled on fused silica surface
    Lagutchev, AS
    Song, KJ
    Huang, JY
    Yang, PK
    Chuang, TJ
    SURFACE & COATINGS TECHNOLOGY, 1997, 94-5 (1-3): : 383 - 389
  • [24] Chemical Imaging of Self-Assembled Monolayers on Copper Using Compressive Hyperspectral Sum Frequency Generation Microscopy
    Zheng, Desheng
    Lu, Liyang
    Kelly, Kevin F.
    Baldelli, Steven
    JOURNAL OF PHYSICAL CHEMISTRY B, 2018, 122 (02): : 464 - 471
  • [25] Chemical imaging of interfaces by sum-frequency generation microscopy: Application to patterned self-assembled monolayers
    Kuhnke, K
    Hoffmann, DMP
    Wu, XC
    Bittner, AM
    Kern, K
    APPLIED PHYSICS LETTERS, 2003, 83 (18) : 3830 - 3832
  • [26] Sum frequency generation spectroscopy of partially fluorinated methyl-terminated self-assembled monolayers on gold and UPD-silver surfaces
    Rodriguez, Daniela
    Marquez, Maria
    Zenasni, Oussama
    Baldelli, Steve
    Lee, T. Randall
    ABSTRACTS OF PAPERS OF THE AMERICAN CHEMICAL SOCIETY, 2018, 255
  • [27] Sum frequency generation spectroscopy probing of formation of self-assembled monolayers from thiols with terminal phenylalanine ring and intrachain amide groups
    Spandyreva, Marija
    Ignatjev, Ilja
    Matulaitiene, Ieva
    Kuodis, Zenonas
    Niaura, Gediminas
    CHEMIJA, 2018, 29 (04): : 219 - 225
  • [28] Sum-frequency generation spectroscopy of self-assembled structures of Guanosine 5′-monophosphate on mica
    Kunstelj, Klemen
    Spindler, Lea
    Federiconi, Francesco
    Bonn, Mischa
    Drevensek-Olenik, Irena
    Copic, Martin
    CHEMICAL PHYSICS LETTERS, 2008, 467 (1-3) : 159 - 163
  • [29] Self-Assembled Film Organization in Fast Microcontact Printing Investigated by Sum Frequency Generation Spectroscopy
    Lis, Dan
    Peremans, Andre
    Sartenaer, Yannick
    Caudano, Yves
    Mani, Alaa Addin
    Dreesen, Laurent
    Thiry, Paul A.
    Guthmuller, Julien
    Champagne, Benoit
    Cecchet, Francesca
    JOURNAL OF PHYSICAL CHEMISTRY C, 2009, 113 (22): : 9857 - 9864
  • [30] Vibrational sum frequency generation spectroscopy of dodecanethiol on metal nanoparticles
    Bordenyuk, Andrey N.
    Weeraman, Champika
    Yatawara, Achani
    Jayathilake, Himali D.
    Stiopkin, Igor
    Liu, Yi
    Benderskii, Alexander V.
    JOURNAL OF PHYSICAL CHEMISTRY C, 2007, 111 (25): : 8925 - 8933
12345