Poly(acrylamide-allyl glycidyl ether) cryogel as a novel stationary phase in dye-affinity chromatography

被引：0
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作者：
Demiryas, Nazan [1 ]
Tüzmen, Nalan [1 ]
Galaev, Igor Yu [2 ]
Pişkin, Erhan [3 ]
Denizli, Adil [4 ]
机构：
[1] Department of Chemistry, Dokuzeylül University, Izmir, Turkey
[2] Department of Biotechnology, Center for Chemistry and Chemical Engineering, Lund University, Lund, Sweden
[3] Department of Chemical Engineering, Hacettepe University, Ankara, Turkey
[4] Department of Chemistry, Hacettepe University, Ankara, Turkey
来源：
Journal of Applied Polymer Science | 2007年 / 105卷 / 04期
关键词：
Poly(acrylamide-allyl glycidyl ether) [poly-(AAm-AGE)] cryogel was prepared by bulk polymerization which proceeds in an aqueous solution of monomers frozen inside a glass column (cryo-polymerization). After thawing; the monolithic cryogel contains a continuous polymeric matrix having interconnected pores of 10-100 μm size. Cibacron Blue F3GA was immobilized by covalent binding onto poly(AAm-AGE) cryogel via epoxy groups. Poly(AAm-AGE) cryogel was characterized by swelling studies; FTIR; scanning electron microscopy; and elemental analysis. The equilibrium swelling degree of the poly-(AAm-AGE) monolithic cryogel was 6.84 g H2O/g cryogel. Poly(AAm-AGE) cryogel containing 68.9 μmol Cibacron Blue F3GA/g was used in the adsorption/desorption of human serum albumin (HSA) from aqueous solutions and human plasma. The nonspecific adsorption of HSA was very low (0.2 mg/g). The maximum amount of HSA adsorption from aqueous solution in acetate buffer was 27 mg/g at pH 5.0. Higher HSA adsorption value was obtained from human plasma (up to 74.2 mg/g). Desorption of HSA with a purity of 92% from Cibacron Blue F3GA attached poly(AAm-AGE) cryogel was achieved using 0.1M Tris/HCl buffer containing 0.5M NaCl. It was observed that HSA could be repeatedly adsorbed and desorbed with poly(AAm-AGE) cryogel without significant loss in the adsorption capacity. © 2007 Wiley Periodicals; Inc;
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页码：1808 / 1816
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