Induced apoptosis and mechanism of endosulfan in mouse germ cells

Endosulfan which is going to be included as POPs in effect of organism have become the focus of international concern. To explore endosulfan on apoptosis of spermatogenic cells and mechanisms, doses of 7.0, 3.5 and 1.75 mg/kg oral administration to mice exposed to 28 consecutive days. Morphology characters of spermatogenic cells observed by laser confocal scanning microscope (LCSM), DNA ladder detected by electricity swims, and increasing of DNA degeneration rate measured through diphenylamine (p less than or equal 0.01). Endosulfan might enhance calcium concentration in the spermatogenic cells and restrained Na+ K+-ATPase and Ca2+ Mg2+-ATPase activities that showed endosulfan might induce spermatogenic cells in the testicle to occur apoptosis (p less than or equal 0.01), to cause the function of transporting Ca2+ to be limited, reduce the ability of excluding calcium and send Ca2+ concentration to hoist sustainingly, i.e. calcium overload in the cells.