Determination of coenzyme Q10 content in raw materials and dietary supplements by high-performance liquid chromatography-UV: Collaborative study

被引：0
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作者：
Lunetta, Steven [1 ]
Roman, Mark [2 ]
Chandrah, A. [2 ]
Edamura, T. [2 ]
Honda, T. [2 ]
LeVanseler, K. [2 ]
Nagarajan, M. [2 ]
Patel, N. [2 ]
Reif, K. [2 ]
Schaneberg, B. [2 ]
Sullivan, D. [2 ]
Trussardo, L. [2 ]
机构：
[1] Pharmavite LLC, 1150 Aviation Pl, San Fernando, CA 91340, United States
[2] Tampa Bay Analytical Research, Inc., 10810 72nd St, Largo, FL 33777, United States
来源：
Journal of AOAC International | 1600年 / 91卷 / 04期
关键词：
An international collaborative study was conducted of a high-performance liquid chromatographic (HPLC)-UV method for the determination of coenzyme Q10 (CoQ10; ubidecarenone) in raw materials and dietary supplements. Ten collaborating laboratories determined the total CoQ10 content in 8 blind duplicate samples. Sample materials included CoQ10 raw material and 4 finished product dietary supplements representing softgels; hardshell gelatin capsules; and chewable wafers. In addition; collaborating laboratories received a negative control and negative control spiked with CoQ10 at low and high levels to determine recovery. Materials were extracted with an acetonitrile - tetrahydrofuran - water mixture. Ferric chloride was added to the test solutions to ensure all CoQ10 was in the oxidized form. The HPLC analyses were performed on a C18 column using UV detection at 275 nm. Repeatability relative standard deviations (RSDr) ranged from 0.94 to 5.05%. Reproducibility relative standard deviations (RSDR) ranged from 3.08 to 17.1%; with HorRat values ranging from 1.26 to 5.17. Recoveries ranged from 74.0 to 115%. Based on these results; the method is recommended for Official First Action for determination of CoQ10 in raw materials and dietary supplement finished products containing CoQ10 at a concentration of >100 mg CoQ10/g test material;
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页码：702 / 708
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