A simple and rapid detection of viral protein using RNA oligonucleotide in a biosensor

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[1] Roh, Changhyun
[2] Kim, Sang-Eun
[3] Jo, Sung-Kee
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Roh, C. | 1600年 / Maik Nauka Publishing / Springer SBM卷 / 67期
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The monitoring of non-structural viral protein 3 (NS3) has been of considerable interest in developing simple and reliable methods for detection of hepatitis C virus (HCV) for applications in diagnostic medicine. Although enzyme-linked immunosorbent assay (ELISA) is the most general method in HCV detection; using antibody brings problems. This method is temperature-sensitive and requires specific reactions condition. In addition; secondary antibody conjugated with enzyme and fluorescent dye is required. To overcome these bottlenecks; we designed a streptavidin-biotin conjugation method; namely; the RNA oligo-nucleotide sensor system that could monitor viral protein with detection limit of 500 pg/mL by using biotin-tagged RNA oligonucleotide in forteBio's Octet optical biosensor system. In this study; we proposed an efficient method for simple and convenient detection of HCV viral protein; with the advantage of target specific monitoring. © 2012 Pleiades Publishing; Ltd;
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