Near-infrared fluorescent probe for visualization of nitroxyl in the plant response to stress

Background: Nitroxyl (HNO) is an emerging signaling molecule that plays a significant regulatory role in various aspects of plant biology, including stress responses and developmental processes. However, understanding the precise actions of HNO in plants has been challenging due to the absence of highly sensitive and real-time in situ monitoring tools. Consequently, it is crucial to develop effective and accurate detection methods for HNO. Establishing such methodologies will enable researchers to elucidate the functional roles of HNO in plant physiological processes, thereby advancing our knowledge of plant resilience and adaptation under environmental stressors.