On the nature of DNA self-assembled monolayers on Au: Measuring surface heterogeneity with electrochemical in situ fluorescence microscopy

被引：0
|
作者：
Murphy, Jeffrey N. [1 ]
Cheng, Alan K. H. [2 ]
Yu, Hua-Zhong [2 ]
Bizzotto, Dan [1 ]
机构：
[1] Advanced Materials and Process Engineering Laboratory, Department of Chemistry, University of British Columbia, Vancouver, BC V6T 1Z4, Canada
[2] Department of Chemistry, Simon Fraser University, Burnaby, BC V5A 1S6, Canada
来源：
Journal of the American Chemical Society | 2009年 / 131卷 / 11期
关键词：
The creation of gold surfaces modified by single-or double-stranded DNA self-assembled monolayers (SAMs) is shown to produce heterogeneous surface packing densities through the use of electrochemical studies coupled with fluorescence imaging. The modified surfaces created by direct adsorption of thiolate DNA [followed by passivation with mecaptohexanol (MCH)] resulted in regions covered by a monolayer of DNA SAM and other regions that were coated by large particles of DNA. The difference in fluorescence intensity measured from these regions was dramatic. More importantly; a regional variance in fluorescence intensity in response to electrochemical potential was observed: the large aggregates showing a significantly different modulation of fluorescence intensity than the monolayer-coated regions. Electrochemical desorption and detection of the fluorescently tagged DNA provided clear evidence of a complete surface modification. These studies have implications for biosensor/biochip development using DNA SAMs. A modification in the method used to produce the DNA SAMs resulted in a significantly different surface with much fewer aggregates and more significant electromodulation of the fluorescence intensity; though at much lower DNA surface density (ca. 1% of maximum theoretical coverage). This method for forming the modified surfaces has clear advantages over the currently accepted practice and emphasizes the importance of studying the nonaveraged nature of the sensor surface using in situ imaging tools like electrofluorescence microscopy. © 2009 American Chemical Society;
D O I：
暂无
中图分类号：
学科分类号：
摘要：
Journal article (JA)
引用
收藏
页码：4042 / 4050
相关论文
共 50 条
  • [21] Electrochemical behaviors of novel electroactive Au nanoparticles protected by self-assembled monolayers
    Sayed Habib Kazemi
    Mojtaba Shamsipur
    Abdolhamid Alizadeh
    Mir Fazlollah Mousavi
    Journal of the Iranian Chemical Society, 2013, 10 : 333 - 338
  • [22] Structure and electrochemical behavior of aromatic thiol self-assembled monolayers on Au(111)
    Noh, J
    Park, H
    Jeong, Y
    Kwon, S
    BULLETIN OF THE KOREAN CHEMICAL SOCIETY, 2006, 27 (03) : 403 - 406
  • [23] ELECTROCHEMICAL POLYMERIZATION OF PYRROLE-CONTAINING SELF-ASSEMBLED ALKANETHIOL MONOLAYERS ON AU
    WILLICUT, RJ
    MCCARLEY, RL
    JOURNAL OF THE AMERICAN CHEMICAL SOCIETY, 1994, 116 (23) : 10823 - 10824
  • [24] DNA Electrochemical Biosensor Based on Au Nanoparticles Self-Assembled Au Electrode
    Liu, Yan
    Dai, Zhao
    Xu, Shichao
    Zhang, Jimei
    Zheng, Guo
    Zhang, Qingyin
    Chen, Guangping
    SOFT MAGNETIC MATERIALS, 2011, 298 : 78 - +
  • [25] Electrochemical assessment of electrochemical oxidation stability of self-assembled monolayers on gold and preparation of binary self-assembled monolayers on gold
    Chen, Yu
    Yang, Chen
    Wang, Feng-Bin
    ELECTROCHIMICA ACTA, 2010, 55 (12) : 3951 - 3956
  • [26] Surface pKa of self-assembled monolayers
    Hale, PS
    Maddox, LM
    Shapter, JG
    Gooding, JJ
    JOURNAL OF CHEMICAL EDUCATION, 2005, 82 (05) : 779 - 781
  • [27] Study of the ferrocenylalkanethiol self-assembled monolayers by electrochemical surface plasmon resonance
    Yao, Xin
    Yang, MinLi
    Wang, Yunfeng
    Hu, Zhongbo
    SENSORS AND ACTUATORS B-CHEMICAL, 2007, 122 (02): : 351 - 356
  • [28] Electrochemical and chemical tuning of the surface wettability of tetrathiafulvalene self-assembled monolayers
    Casado-Montenegro, Javier
    Mas-Torrent, Marta
    Oton, Francisco
    Crivillers, Nuria
    Veciana, Jaume
    Rovira, Concepcio
    CHEMICAL COMMUNICATIONS, 2013, 49 (73) : 8084 - 8086
  • [29] Review-Electrical Manipulation of DNA Self-Assembled Monolayers: Electrochemical Melting of Surface-Bound DNA
    West, Ryan M.
    JOURNAL OF THE ELECTROCHEMICAL SOCIETY, 2020, 167 (03)
  • [30] Electrochemical in-situ Raman study of azobenzene self-assembled monolayers on gold
    Zhu, T
    Yu, HZ
    Cai, SM
    Liu, ZF
    MOLECULAR CRYSTALS AND LIQUID CRYSTALS SCIENCE AND TECHNOLOGY SECTION A-MOLECULAR CRYSTALS AND LIQUID CRYSTALS, 1997, 294 : 79 - 82
12345