Environmental monitoring of Listeria monocytogenes contamination in dairy processing facilities combining culturing technique and molecular methods

被引：0

作者：

Salza, S. ^{[1
]}

Piras, G. ^{[1
]}

Melillo, R. ^{[1
]}

Molotzu, M. ^{[1
]}

Giagnoni, L. ^{[2
]}

Doneddu, L. ^{[2
]}

Tondello, A. ^{[3
]}

Cecchinato, A. ^{[3
]}

Stevanato, P. ^{[3
]}

Squartini, A. ^{[3
]}

Tedde, T. ^{[1
]}

Virgilio, S. ^{[1
]}

Mudadu, A.G. ^{[1
]}

Spanu, C. ^{[2
]}

机构：

[1] Veterinary Public Health Institute of Sardinia, Complex Structure of Food Hygiene, Via Duca degli Abruzzi 8, Sassari,07100, Italy

[2] Department of Veterinary Medicine, University of Sassari, Via Vienna 2, Sassari,07100, Italy

[3] Department of Agronomy, Food, Natural Resources, Animals and Environment, DAFNAE, University of Padova, Viale, dell'Università 16, PD, Legnaro,35020, Italy

来源：

LWT | 2024年 / 211卷

关键词：

D O I：

10.1016/j.lwt.2024.116870

中图分类号：

学科分类号：

摘要：

Objective: of the present study was to investigate Listeria contamination in Sardinian sheep cheese-making plants using culture and DNA-based methods. Food and environmental samples were collected from different surfaces of 14 facilities. Samples were collected using pre-moistened sponge swabs. Each site was sampled using two sponges used one for microbiological detection and the other for extraction of total DNA. The DNA was submitted to real time PCR targeting the prs gene for Listeria spp., the gene inlA for Listeria monocytogenes and the gene iap for Listeria innocua. DNA metabarcoding was also conducted on the total DNA. Overall were collected 254 environmental and 36 food samples. The prevalence of Listeria spp. and Listeria monocytogenes was 18.6% and 10.0%, respectively. Samples enrichment coupled with quantitative PCR (qPCR) showed a greater sensitivity than the conventional culture method with an overall prevalence of 12.1% for L. monocytogenes. Both, qPCR and ribosomal DNA-metabarcoding conducted on total DNA extracted from the sponges performed poorly in comparison to the culture-based method detecting the presence of Listeria genus in as little as 7 (2.4%) and 4 (1.4%) samples respectively. The present study supports the use of a culture enrichment coupled with qPCR for routine monitoring of contamination in food premises. © 2024 The Authors

引用

共 32 条

[31] Molecular and Phenotypic Characterization of Listeria monocytogenes from US Department of Agriculture Food Safety and Inspection Service Surveillance of Ready-to-Eat Foods and Processing Facilities
Ward, Todd J.
Evans, Peter
Wiedmann, Martin
Usgaard, Thomas
Roof, Sherry E.
Stroika, Steven G.
Hise, Kelley
JOURNAL OF FOOD PROTECTION, 2010, 73 (05) : 861 - 869
[32] Intensive Environmental Sampling and Whole Genome Sequence-based Characterization of Listeria in Small- and Medium-sized Dairy Facilities Reveal Opportunities for Simplified and Size-appropriate Environmental Monitoring Strategies
Bolten, Samantha
Lott, Timothy T.
Ralyea, Robert D.
Gianforte, Anika
Trmcic, Aljosa
Orsi, Renato H.
Martin, Nicole H.
Wiedmann, Martin
JOURNAL OF FOOD PROTECTION, 2024, 87 (04)

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