Intervention Effect of Chitooligosaccharide on Alcohol-Induced Intestinal Damage in Rats

被引：0
|
作者：
Xu Y. [1 ,2 ]
Wang B. [1 ,2 ]
Jiang Q. [1 ,2 ]
Xia W. [1 ,2 ]
Xu Y. [1 ,2 ]
机构：
[1] School of Food Science and Technology, Jiangnan University, Wuxi
[2] Collaborative Innovation Center of Food Safety and Quality Control in Jiangsu Province, Wuxi
来源：
Shipin Kexue/Food Science | 2021年 / 42卷 / 03期
关键词：
Alcohol; Chitooligosaccharide; Inflammatory response; Intestinal mucosal barrier; Oxidative damage;
D O I：
10.7506/spkx1002-6630-20191229-340
中图分类号：
学科分类号：
摘要：
Objective: To investigate the intervention effect of chitooligosaccharide (COS) on intestinal injury induced by chronic drinking.Methods: Male Wistar rats were randomly divided into five groups: blank control, model, low-, medium-, and high-dose COS treatment groups.The rats in all groups except the blank control group were intragastrically administered with 4.5 g/kg mb of alcohol for 42 successive days to induce intestinal injury, which was preceded by COS for the treatment groups.Results: COS could significantly improve the performance of the rat duodenal smooth muscle(P < 0.05), restore small intestinal health, significantly reduce the concentration of D-lactic acid (D-LA), diamine oxidase(DAO) activity, the levels of pro-inflammatory cytokines including interleukin (IL)-1β, tumor necrosis factor (TNF)-α and IL-6, malondialdehyde (MDA) concentration and protein carbonyl content in plasma (P < 0.05) and claudin-4 gene expression (P < 0.05), and significantly inhibit the gene expression of claudin-4 (P < 0.05).High-dose COS could significantly increase the gene expression of the tight junction proteins occludin and ZO-1 (P < 0.05).Medium- and high-dose COS could significantly reduce gene the expression of IL-6, IL-1β and TNF-α in the duodenum (P < 0.05).Conclusion: COS alleviates intestinal damage caused by chronic drinking in rats by improving small intestinal health, enhancing intestinal mucosal barrier, reducing inflammatory responses and alleviating oxidative damage. © 2021, China Food Publishing Company. All right reserved.
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页码：158 / 164
页数：6
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