Analysis of 21 sunscreen agents in cosmetics with quantitative analysis of multi-components by single marker

A quantitative analysis of multi-components by single marker （QAMS）method for the determination of 21 kinds of sunscreen agents in cosmetics was established. Using methanol，tetrahydrofuran and perchloric acid as mobile phases，21 kinds of sunscreen agents were separated on a C18 column and detected by high performance liquid chromatography（HPLC）with gradient elution. Diphenylketone-3 was selected as the internal reference substance，and the relative correction factors of the other 20 kinds of sunscreen agents were established to calculate the content of each component. The results showed that the 21 kinds of sunscreen agents had good linear relationships within a certain mass concentration ranges，and each component was stable for 24 h. The results of QAMS were close to that of external standard method（ESM），and the relative error was not higher than 4.25%. This method can be used for the determination and quality control of sunscreen agents in cosmetics. © 2023, Youke Publishing Co.,Ltd. All rights reserved.