Restoration of soil multifunctional indicators requires more than thirty years in degraded shrubland of a semi-arid mountainous ecosystem

Vegetation degradation in natural environments leads to considerable fluctuations in soil function indicators, particularly in the sensitive and delicate habitats of semi-arid regions. In this study, the dynamic of both litter and soil properties was examined in northern Iran, in sites with Crataegus melanocarpa and Berberis integerrima dominance. The chosen sites have been degraded in 1993 due to exploitation for fuel production. Litter and soil (0-10, 10-20 and 20-30 cm depths) samples were collected in sites that have been degraded [i.e., 10-years post- degradation (2003), 20-years post-degradation (2013), and 30-years post-degradation (2023)]. A site not to exposed to degradation (year 2023) was also included as a control. In total, 48 litter samples (4 study sites x 12 samples) and 144 soil samples (4 study sites x 3 depths x 12 samples) were collected. Our findings showed a significant reduction of approximately 60-70% in the chemical characteristics of the litter, such as phosphorus (P) and magnesium (Mg) after 10-years post-degradation (2003) compared to the control site. Soil fertility indicators were also reduced 2-6 times as a result of habitat degradation. Aligned with this, the biota population in the 10-year post-degradation site showed a decrease of 70-90% with respect to the control. Moreover, the densities of fungi and bacteria decreased by approximately 30%-70% and 45-80% respectively compared to the 30-year post-degraded site. The peak activation of soil enzymes including urease, acid phosphatase, arylsulfatase, and invertase was found in the site that was not subjected to degradation, being 3.5, 5.8, 6.7 and 6.7- times higher in comparison to the 10-year post-degradation site. Overall, the results showed that in the non-degraded ecosystems, the input of plant-based organic matter was accompanied by an increase in soil fertility, organism population and soil function. This pinpoints the importance of protecting plant species in these areas, while in degraded areas planting native species adapted to the region's climate and fertilization could significantly contribute to restoring these areas.