Antimicrobial adhesive self-healing hydrogels for efficient dental biofilm removal from periodontal tissue

被引:1
|
作者
Kim, Han Sol [1 ]
Kim, Minkyoung [2 ]
Kim, Youngjoon [2 ]
Shin, Hyun Ho [3 ]
Lee, Sang-woo [2 ,4 ,6 ]
Ryu, Ji Hyun [1 ,3 ,5 ,7 ]
机构
[1] Wonkwang Univ, Dept Carbon Convergence Engn, Iksan 54538, South Korea
[2] Seoul Natl Univ, Dent Res Inst, Dept Physiol & Neurosci, Sch Dent, Seoul 03080, South Korea
[3] Wonkwang Univ, Dept Chem Engn, Iksan 54538, South Korea
[4] Inst Basic Sci IBS, Ctr Nanoparticle Res, Seoul 08826, South Korea
[5] Wonkwang Univ, Smart Convergence Mat Anal Ctr, Iksan 54538, Jeonbuk, South Korea
[6] Seoul Natl Univ, Sch Dent & Dent, Dept Physiol, Res Inst, Seoul 110460, South Korea
[7] Wonkwang Univ, Smart Convergence Mat Anal Ctr, Dept Carbon Convergence Engn, Iksan, Jeonbuk, South Korea
基金
新加坡国家研究基金会;
关键词
Periodontitis; Peri-implantitis; Alginate; Phenylboronic acid; Tannic acid; Antimicrobial; Self-healing; Tissue adhesiveness; Porphyromonas gingivalis; Hydrogel; TANNIC-ACID; ANTIOXIDANT; GEL;
D O I
10.1016/j.dental.2024.09.012
中图分类号
R78 [口腔科学];
学科分类号
1003 ;
摘要
Objectives: Oral biofilms, including pathogens such as Porphyromonas gingivalis, are involved in the initiation and progression of various periodontal diseases. However, the treatment of these diseases is hindered by the limited efficacy of many antimicrobial materials in removing biofilms under the harsh conditions of the oral cavity. Our objective is to develop a gel-type antimicrobial agent with optimal physicochemical properties, strong tissue adhesion, prolonged antimicrobial activity, and biocompatibility to serve as an adjunctive treatment for periodontal diseases. Methods: Phenylboronic acid-conjugated alginate (Alg-PBA) was synthesized using a carbodiimide coupling agent. Alg-PBA was then combined with tannic acid (TA) to create an Alg-PBA/TA hydrogel. The composition of the hydrogel was optimized to enhance its mechanical strength and tissue adhesiveness. Additionally, the hydrogel's self-healing ability, erosion and release profile, biocompatibility, and antimicrobial activity against P. gingivalis were thoroughly characterized. Results: The Alg-PBA/TA hydrogels, with a final concentration of 5 wt% TA, exhibited both mechanical properties comparable to conventional Minocycline gel and strong tissue adhesiveness. In contrast, the Minocycline gel demonstrated negligible tissue adhesion. The Alg-PBA/TA hydrogel also retained its rheological properties under repeated 5 kPa stress owing to its self-healing capability, whereas the Minocycline gel showed irreversible changes in rheology after just one stress cycle. Additionally, Alg-PBA/TA hydrogels displayed a sustained erosion and TA release profile with minimal impact on the surrounding pH. Additionally, the hydrogels exhibited potent antimicrobial activity against P. gingivalis, effectively eliminating its biofilm without compromising the viability of MG-63 cells. Significance: The Alg-PBA/TA hydrogel demonstrates an optimal combination of mechanical strength, selfhealing ability, tissue adhesiveness, excellent biocompatibility, and sustained antimicrobial activity against P. gingivalis. These attributes make it superior to conventional Minocycline gel. Thus, the Alg-PBA/TA hydrogel is a promising antiseptic candidate for adjunctive treatment of various periodontal diseases.
引用
收藏
页码:1970 / 1980
页数:11
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