Bacterial Diversity of Crayfish from Different Origins by Traditional Culture-dependent Method and Metagenome Sequencing

被引：0

作者：

Tang C. ^{[1
]}

Liu F. ^{[1
]}

Zhu Y. ^{[1
]}

Wu H. ^{[1
]}

Sun Z. ^{[1
]}

机构：

[1] Institute of Agricultural Products Processing, Jiangsu Academy of Agricultural Sciences, Nanjing

来源：

Journal of Chinese Institute of Food Science and Technology | 2021年 / 21卷 / 02期

关键词：

Bacterial diversity; Culture-dependent method; Metagenomics sequencing; Procambarus clarkii;

D O I：

10.16429/j.1009-7848.2021.02.032

中图分类号：

学科分类号：

摘要：

The difference of initial microbial communities of crayfish (Procambarus clarkii) from different origins of Pukou and Dongshan was studied using culture-dependent method and metagenomic analysis in this study. The results showed that the initial total bacterial counts of whole crayfish samples of Pukou and Dongshan was not significant different, but the bacterial counts on the selective enrichment cultures were not significant different. It was found that Aeromonas and Citrobacter were the dominant bacteria in the total samples of Pukou crayfish, Hafnia paralvei was the domain bacterium in the total samples of Dongshan crayfish by 16S rRNA gene analysis. At family level, Enterobacteriaceae strains were the domain bacteria of the total samples from these two regions. At genus level, Aeromonas and Exiguobacterium were dominant bacteria in total samples from Pukou crayfish, and Flavobacterium, Aeromonas and Shewanella were the domain bacteria in the total samples of Dongshan crayfish. The results of the initial bacterial diversity of different parts of crayfish (Procambarus clarkii) from different origins indicated that the potential food safety risk of crayfish, and provided a reference for the future study of crayfish preservation and transportation. © 2021, Editorial Office of Journal of CIFST. All right reserved.

引用

页码：260 / 268

页数：8

共 27 条

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