Supercritical fluid extraction of ergosterol from fermented Ganoderma lucidum

Supercritical fluid extraction conditions of ergosterol from Ganoderma lucidum after fermentation were optimization by orthogonal test on the basis of single factor experiment. The results showed that the extraction pressure and temperature were significant factors and the optimization conditions were as follows: extraction pressure 20 MPa, extraction temperature 50°C, and CO2 flow rate 2.5 L/min, under which the content of ergosterol reached 1.1362 mg/g.