Biodiesel production by immobilized lipase

被引:0
|
作者
Nie, Kai-Li [1 ]
Wang, Fang [1 ]
Tan, Tian-Wei [1 ]
机构
[1] Key Lab. of Bioprocess, Dept. of Biochem. Eng., Beijing Univ. of Chem. Technol., Beijing 100029, China
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关键词
Bioconversion - Biotechnology - Enzyme immobilization - Enzymes - Esterification - Esters - Fatty acids - Methanol;
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学科分类号
摘要
A Candida sp.99-125 lipase prepared by the authors was used to catalyze transesterification and was studied with the effects of the methanol toxicity, enzyme amount, solvent, water content, pH, temperature, reaction time and molar ratio of the substrate on the reaction, and the problem about the life-span of the immobilized lipase was involved as well. The results show that the transesterification degree under the optimal parameters and process can reach 96%, and the half-life of the immobilized lipase can be longer than 200 h.
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页码:35 / 38
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