Aluminum effect on Eucalyptus grandis x E. urophylla shoots growth in vitro

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作者
Basso, Lúcia Helena Menegon [1 ]
Gonçalves, Antonio Natal [2 ]
Vaz de Arruda Silveira, Liciana [3 ]
Lima, Giuseppina Pace Pereira [4 ]
机构
[1] Depto. de Ciências Florestais, ESALQ/USP, Superv. Admin. Polo Capacitacao E.B., Caixa Postal 545, Botucatu, SP - 18618-000, Brazil
[2] Depto. de Ciências Florestais, ESALQ / USP, Caixa Postal 9, Piracicaba, SP 13400-970, Brazil
[3] Depto. de Bioestatística, IB/UNESP, Caixa Postal 510, Botucatu, SP 18618-000, Brazil
[4] Departamento de Química, IB/UNESP, Caixa Postal 545, 18618-000 - Botucatu, SP, Brazil
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关键词
Aluminum - Cloning - Plant cell culture - Proteins;
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摘要
The aim of this work was to evaluate the effects of aluminum on the growth of Eucalyptus shoots cultivated in vitro through nutrient and total soluble protein content. The trial had a totally randomized design with four treatments and four replicates. The treatments were: 0.0; 0.25; 0.5 and 1.0 mM of AlCl 3.6H2O. Shoots without roots of a Eucalyptus grandis x E urophylla clone were used for the in vitro culture. Evaluations were made on the 4th, 8th, 12th, 16th, 20th, 24th and 28th day of culture. The Al addition to the culture media reduced mainly Ca, P and K availability and absorptions by the shoots. The cellular metabolism was affected, conducted to morphological alterations in shoots (browning, mass calluses formation and shoots not friable), dry matter increased and a decreased in total protein soluble.
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页码:167 / 177
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