In order to improve the aesthetic qualities of expressway corridor and enhance the operability of landscape planning, the properties of natural landscape resources was analyzed in this paper. Besides, according to the sequentiality of natural landscape resources along the corridor, optimal segmentation of ordered samples (OSOS) was proposed to divide natural landscape segment of expressway. Through experiments of driving monotony and intervals of landscape nodes, the interval formula was deduced to calculate the cultural landscape node position. In the case of Xi'an-Baoji expressway, the research method was adopted to classify the natural landscape segments, calculate their node positions and determine the theme of each segment. The results show that the OSOS method is consistent with engineering practice, is easy to operate and has strong practicality. The interval distance between cultural landscape nodes should be V85/12 kilometers and the design of landscape elements or landscape sketches should take nodes as the control nodes and be dominated by the themes of landscape segments. The method of OSOS and node control can provide practical guidance for expressway landscape segment planning and nodes design. ©, 2015, Editorial Department of Journal of Chang'an University (Natural Science Edition). All right reserved.