Transformation system of Beauveria bassiana and Metarhizium anisopliae using nitrate reductase gene of Aspergillus nidulans

被引：0
|
作者：
Sandhu, S.S. [1 ]
Kinghorn, J.R. [1 ]
Rajak, R.C. [1 ]
Unkles, S.E. [1 ]
机构：
[1] Department of Biological Science, Rani Durgawati University, Jabalpur 482001, India
来源：
| 2001年 / National Institute of Science Communication卷 / 39期
关键词：
Biochemistry - Cloning - DNA - Enzyme kinetics - Mutagenesis;
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学科分类号：
摘要：
An heterologous transformation system for entomopathogenic fungi B. bassiana and M. anisopliae was developed based on the use of A. nidulans nitrate reductase gene (niaD). B. bassiana and M. anisopliae niaD stable mutants were selected by treatment of protoplast with ethane methane sulphonate (EMS) and regenerated on chlorate medium. The cloned gene was capable of transforming B. bassiana and M. anisopliae at a frequency of 5.8 to 20 transformants per μg of DNA. Most of them were mitotically stable.
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