Transformation system of Beauveria bassiana and Metarhizium anisopliae using nitrate reductase gene of Aspergillus nidulans

被引：0
|
作者：
Sandhu, S.S. [1 ]
Kinghorn, J.R. [1 ]
Rajak, R.C. [1 ]
Unkles, S.E. [1 ]
机构：
[1] Department of Biological Science, Rani Durgawati University, Jabalpur 482001, India
来源：
| 2001年 / National Institute of Science Communication卷 / 39期
关键词：
Biochemistry - Cloning - DNA - Enzyme kinetics - Mutagenesis;
D O I：
暂无
中图分类号：
学科分类号：
摘要：
An heterologous transformation system for entomopathogenic fungi B. bassiana and M. anisopliae was developed based on the use of A. nidulans nitrate reductase gene (niaD). B. bassiana and M. anisopliae niaD stable mutants were selected by treatment of protoplast with ethane methane sulphonate (EMS) and regenerated on chlorate medium. The cloned gene was capable of transforming B. bassiana and M. anisopliae at a frequency of 5.8 to 20 transformants per μg of DNA. Most of them were mitotically stable.
引用
收藏
相关论文
共 50 条
  • [1] Characterization of Beauveria bassiana, Metarhizium anisopliae and Aspergillus nidulans through electrophoretic patterns of their protein fractions
    Gorinstein, S
    PaccolaMeirelles, LD
    Belo, VA
    Azevedo, JL
    JOURNAL OF FERMENTATION AND BIOENGINEERING, 1996, 82 (02): : 89 - 92
  • [2] Whey for mass production of Beauveria bassiana and Metarhizium anisopliae
    Kassa, Adane
    Brownbridge, Michael
    Parker, Bruce L.
    Skinner, Margaret
    Gouli, Vladimir
    Gouli, Svetlana
    Guo, Mingruo
    Lee, Frank
    Hata, Teri
    MYCOLOGICAL RESEARCH, 2008, 112 : 583 - 591
  • [3] Sporulation of Metarhizium anisopliae and Beauveria bassiana on Coptotermes formosanus and in vitro
    Sun, JZ
    Fuxa, JR
    Henderson, G
    JOURNAL OF INVERTEBRATE PATHOLOGY, 2002, 81 (02) : 78 - 85
  • [4] Comparison of pathogenicities of Beauveria bassiana and Metarhizium anisopliae to chestnut pests
    Ihara, Fumio
    Toyama, Masatoshi
    Higaki, Morio
    Mishwo, Koji
    Yaginuma, Katsuhiko
    APPLIED ENTOMOLOGY AND ZOOLOGY, 2009, 44 (01) : 127 - 132
  • [5] Tropical Occurrence and Agricultural Importance of Beauveria bassiana and Metarhizium anisopliae
    McGuire, Amy V.
    Northfield, Tobin D.
    FRONTIERS IN SUSTAINABLE FOOD SYSTEMS, 2020, 4
  • [6] Spatial dispersal of Metarhizium anisopliae and Beauveria bassiana in soybean fields
    Guinossi, Heverly das Merces
    Moscardi, Flavio
    Neves de Oliveira, Maria Cristina
    Sosa-Gomez, Daniel Ricardo
    TROPICAL PLANT PATHOLOGY, 2012, 37 (01) : 44 - 49
  • [7] Pathogenicity of Beauveria bassiana and Metarhizium anisopliae against Galleria mellonella
    Khalid A. Hussein
    Mohamed A. A. Abdel-Rahman
    Ahmed Y. Abdel-Mallek
    Saad S. El-Maraghy
    Jin Ho Joo
    Phytoparasitica, 2012, 40 : 117 - 126
  • [8] Efficacy of Beauveria bassiana and Metarhizium anisopliae against wheat aphid
    Saif, Iqra
    Sufyan, Muhammad
    Baboo, Irfan
    Jabbar, Muhammad
    Shafiq, Ahsan
    Saif, Rida Nosheen
    Liaqat, Umer
    Lackner, Maximilian
    EUROBIOTECH JOURNAL, 2024, 8 (01): : 23 - 31
  • [9] Virulence of Metarhizium anisopliae and Beauveria bassiana to Ctenocephalides felis felis
    de Melo, Denise R.
    Fernandes, Everton K. K.
    da Costa, Gisela L.
    Scott, Fabio B.
    Bittencourt, Vania R. E. P.
    ANIMAL BIODIVERSITY AND EMERGING DISEASES: PREDICTION AND PREVENTION, 2008, 1149 : 388 - 390
  • [10] Pathogenicity of Beauveria bassiana and Metarhizium anisopliae against Galleria mellonella
    Hussein, Khalid A.
    Abdel-Rahman, Mohamed A. A.
    Abdel-Mallek, Ahmed Y.
    El-Maraghy, Saad S.
    Joo, Jin Ho
    PHYTOPARASITICA, 2012, 40 (02) : 117 - 126
12345