Determination and Comparison of α-Carotene and β-Carotene of Plant-Derived Foods

被引：0
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作者：
机构：
[1] [1,Cui, Yajuan
[2] Kong, Fanhua
[3] Liu, Guojie
[4] Lu, Fei
[5] Li, Dong
来源：
Liu, Guojie (lgj@cau.edu.cn) | 2018年 / Chinese Chamber of Commerce卷 / 39期
关键词：
D O I：
10.7506/spkx1002-6630-201804029
中图分类号：
学科分类号：
摘要：
The study was conducted to develop an analytical method for simultaneous online determination of α-carotene and β-carotene in plant-derived foods by high performance liquid chromatography (HPLC). Furthermore, the method was validated with freeze-dried carrot powder. The results showed that the calibration curves for α-carotene and β-carotene were linear in the ranges of 0.8-20 and 0.4-8.2 μg/mL, respectively. The precision expressed as relative standard deviation (RSD) were 4.45% and 4.71%, respectively. The recoveries at three spiked levels were 96%-112% and 83.5%-103% with RSD values of 2.67%-5.83% and 4.90%-7.42%, respectively. These data indicated good repeatability and accuracy. Comparative analysis of various plant-derived foods by this method showed that β-carotene was more abundant than α-carotene in all samples except for pumpkin, which gave the opposite result. © 2018, China Food Publishing Company. All right reserved.
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