Synthesis and characterization of a amphiphilic pluronic-poly(D,L-lactide- co-glycolide) copolymer and their nanoparticles as protein delivery systems

被引：0
|
作者：
Wu, Yan [1 ]
Che, Fu-Bin [2 ]
Chen, Jiang-Han [2 ]
机构：
[1] National Center for Nanoscience and Technology, Department of Nano-medicine, Nano-biotech Laboratory, Beijing 100080, China
[2] Shanghai Changzheng Hospital, Department of Pharmaceutics, Shanghai 200003, China
来源：
Journal of Applied Polymer Science | 2008年 / 110卷 / 02期
关键词：
A new amphiphilic Pluronic (F68)-PLGA copolymer; which can be used to prepare the stealth or long-circulating nanoparticles; was synthesized with Pluronic (F68); Di-Lactide; and glycolide. The structures of Pluronic (F68)-PLGA copolymer as the products were characterized with infrared spectrometry; nuclear magnetic resonance and their molecular weights were determined by gel permeation chromatography. Two methods; double emulsion (DE) and nanoprecipitation (NP); were employed to fabricate the polymeric nanoparticles. Bovine serum albumin (BSA) was loaded into nanoparticles as a model protein. Influence of the preparation conditions on the nanoparticles size; encapsulation efficiency; and release profile in vitro was investigated. They showed the entrapment efficiency of 42.9-63.4% and the average diameter of 119.1-342.8 nm depending on the fabrication technique of nanoparticles and the type of copolymer. The stability maintenance of BSA in the nanoparticle release in vitro was also measured via sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE); circular dichroism (CD); and fluorescence spectrometry. The results showed that the new copolymer could load BSA effectively and BSA kept stable after it was released from the nanoparticles. © 2008 Wiley Periodicals; Inc;
D O I：
暂无
中图分类号：
学科分类号：
摘要：
Journal article (JA)
引用
收藏
页码：1118 / 1128
相关论文
共 50 条
  • [41] Conjugated poly(D,L-lactide-co-glycolide) for the preparation of in vivo detectable nanoparticles
    Tosi, G
    Rivasi, F
    Gandolfi, F
    Costantino, L
    Vandelli, MA
    Forni, F
    BIOMATERIALS, 2005, 26 (19) : 4189 - 4195
  • [42] In vitro degradation of poly (D, L-lactide-co-glycolide) nanoparticles loaded with linamarin
    Hussein, Ahmed S.
    Abdullah, Norhafizah
    Ahmadun, Fakhru'l-Razi
    IET NANOBIOTECHNOLOGY, 2013, 7 (02) : 33 - 41
  • [43] Poly (D,L-lactide-co-glycolide) nanoparticles: Uptake by epithelial cells and cytotoxicity
    Nkabinde, L. A.
    Shoba-Zikhali, L. N. N.
    Semete-Makokotlela, B.
    Kalombo, L.
    Swai, H.
    Grobler, A.
    Hamman, J. H.
    EXPRESS POLYMER LETTERS, 2014, 8 (03): : 197 - 206
  • [44] Characterization of biodegradable poly(D,L-lactide-co-glycolide) nanoparticles loaded with bacteriochlorophyll-a for photodynamic therapy
    Gomes, Anderson J.
    Lunardi, Claure N.
    Tedesco, Antonio C.
    PHOTOMEDICINE AND LASER SURGERY, 2007, 25 (05) : 428 - 435
  • [45] Formulation and Characterization of Poly (D, L-Lactide-Co-glycolide) Nanoparticles Loaded with Achyranthes aspera for Increasing Bioavailability
    Sangal, Aditi
    Rattan, Sunita
    ADVANCES IN POLYMER SCIENCES AND TECHNOLOGY (APA 2017), 2018, : 187 - 195
  • [46] Poly(lactide-co-glycolide) Nanoparticles for an Extended Delivery of Bevacizumab to Retina: Formulation and In Vitro Characterization
    Pandit, Jayamanti
    Sultana, Yasmin
    Aqil, Mohd.
    ADVANCED SCIENCE LETTERS, 2014, 20 (7-9) : 1588 - 1593
  • [47] Conjugates of poly(DL-lactide-co-glycolide) on amino cyclodextrins and their nanoparticles as protein delivery system
    Gao, Hui
    Wang, Yi-Nong
    Fan, Yun-Ge
    Ma, Jian-Biao
    JOURNAL OF BIOMEDICAL MATERIALS RESEARCH PART A, 2007, 80A (01) : 111 - 122
  • [48] Comparative assessment of plasmid DNA delivery by encapsulation within or adsorbed on poly (D, L-lactide-co-glycolide) nanoparticles
    Doolaanea, Abd Almonem
    Mansor, Nur Izzati
    Nor, Nurul Hafizah Mohd
    Shafri, Mohd Affendi Bin Mohd
    Mohamed, Farahidah
    TROPICAL JOURNAL OF PHARMACEUTICAL RESEARCH, 2018, 17 (01) : 1 - 10
  • [49] Design of surface-modified poly(D,L-lactide-co-glycolide) nanoparticles for targeted drug delivery to bone
    Choi, Sung-Wook
    Kim, Jung-Hyun
    JOURNAL OF CONTROLLED RELEASE, 2007, 122 (01) : 24 - 30
  • [50] Delivery of phytochemical thymoquinone using molecular micelle modified poly(D, L lactide-co-glycolide) (PLGA) nanoparticles
    Ganea, Gabriela M.
    Fakayode, Sayo O.
    Losso, Jack N.
    van Nostrum, Cornelus F.
    Sabliov, Cristina M.
    Warner, Isiah M.
    NANOTECHNOLOGY, 2010, 21 (28)
12345