Synthesis and characterization of a amphiphilic pluronic-poly(D,L-lactide- co-glycolide) copolymer and their nanoparticles as protein delivery systems

被引：0
|
作者：
Wu, Yan [1 ]
Che, Fu-Bin [2 ]
Chen, Jiang-Han [2 ]
机构：
[1] National Center for Nanoscience and Technology, Department of Nano-medicine, Nano-biotech Laboratory, Beijing 100080, China
[2] Shanghai Changzheng Hospital, Department of Pharmaceutics, Shanghai 200003, China
来源：
Journal of Applied Polymer Science | 2008年 / 110卷 / 02期
关键词：
A new amphiphilic Pluronic (F68)-PLGA copolymer; which can be used to prepare the stealth or long-circulating nanoparticles; was synthesized with Pluronic (F68); Di-Lactide; and glycolide. The structures of Pluronic (F68)-PLGA copolymer as the products were characterized with infrared spectrometry; nuclear magnetic resonance and their molecular weights were determined by gel permeation chromatography. Two methods; double emulsion (DE) and nanoprecipitation (NP); were employed to fabricate the polymeric nanoparticles. Bovine serum albumin (BSA) was loaded into nanoparticles as a model protein. Influence of the preparation conditions on the nanoparticles size; encapsulation efficiency; and release profile in vitro was investigated. They showed the entrapment efficiency of 42.9-63.4% and the average diameter of 119.1-342.8 nm depending on the fabrication technique of nanoparticles and the type of copolymer. The stability maintenance of BSA in the nanoparticle release in vitro was also measured via sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE); circular dichroism (CD); and fluorescence spectrometry. The results showed that the new copolymer could load BSA effectively and BSA kept stable after it was released from the nanoparticles. © 2008 Wiley Periodicals; Inc;
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页码：1118 / 1128
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