Dynamic full-field optical coherence tomography: 3D live-imaging of retinal organoids

被引:0
|
作者
Scholler, Jules [1 ]
Groux, Kassandra [1 ]
Goureau, Olivier [2 ]
Sahel, José-Alain [2 ,3 ,4 ,5 ]
Fink, Mathias [1 ]
Reichman, Sacha [2 ]
Boccara, Claude [1 ]
Grieve, Kate [2 ,3 ]
机构
[1] Institut Langevin, ESPCI Paris, PSL University, CNRS, 10 rue Vauquelin, Paris, France
[2] Institut de la Vision, Sorbonne Université, INSERM, CNRS, Paris,F-75012, France
[3] Quinze-Vingts National Eye Hospital, 28 Rue de Charenton, Paris,75012, France
[4] Fondation Ophtalmologique Rothschild, Paris,F-75019, France
[5] Department of Ophthalmology, The University of Pittsburgh School of Medicine, Pittsburgh,PA,15213, United States
来源
Light: Science and Applications | 2020年 / 9卷 / 01期
基金
欧洲研究理事会;
关键词
Cell proliferation - Ophthalmology - Stem cells - Tissue;
D O I
暂无
中图分类号
学科分类号
摘要
Optical coherence tomography offers astounding opportunities to image the complex structure of living tissue but lacks functional information. We present dynamic full-field optical coherence tomography as a technique to noninvasively image living human induced pluripotent stem cell-derived retinal organoids. Coloured images with an endogenous contrast linked to organelle motility are generated, with submicrometre spatial resolution and millisecond temporal resolution, creating a way to identify specific cell types in living tissue via their function. © 2020, The Author(s).
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