The study quantitatively assesses the hydrological alterations induced by the Luv Kush Barrage on the Ganga River, India, using the Range of Variability Approach (RVA) to analyze changes in flow conditions before and after the barrage construction. The Ganga River, a crucial water source for millions, has experienced significant hydrological changes due to human interventions, notably the construction of the Luv Kush Barrage. Employing indicators of hydrological alteration (IHAs), we compared pre-impact (1972–1994) and post-impact (2001–2014) flow data to ascertain the barrage’s effects on the river’s flow regime. Our findings reveal significant alterations in the magnitude of monthly streamflow, especially during the monsoon season, with notable decreases in July, August, and September. Alterations were observed in the annual extreme flow conditions, frequency, and duration of high and low pulses, indicating a comprehensive impact on the river’s natural flow patterns. These alterations affect the river’s ecological health, affecting aquatic habitats, fishery resources, and community livelihoods dependent on the river. The study underscores the necessity for integrated water resource management practices that consider the ecological and socio-economic implications of hydrological alterations. © 2024 Indian Society for Hydraulics.